A kind of Taro ssr primer set and its application

Abstract

The present invention relates to the technical field of molecular markers, in particular to a taro SSR primer set and its application. The present invention uses taro as a material, extracts taro DNA, performs enzymatic digestion, sequencing, and analysis to obtain corresponding SSR primers, which can be used for the heredity of taro The applied research on diversity and genetic relationship provides more effective SSR markers. After analysis, this primer has the characteristics of high polymorphic percentage, and the effect of using this primer to verify and amplify is better; the choice of this primer set is Taro in genetic breeding It provides valuable genetic tools for research such as QTL analysis, phylogenetic relationship identification, genetic diversity research and heterosis prediction, and through the study of the genetic relationship between pedigree parents, it can be used for the rational selection of breeding parents of taro and the innovation of taro germplasm. Use to provide reference.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular markers, in particular to a taro SSR primer set and its application. 【Background technique】 [0002] Taro, also known as taro, taro, is the underground bulb of the Araceae plant. The shape and flesh quality vary according to the variety. It is a perennial tuber plant and is often cultivated as an annual crop. my country's taro germplasm resources are extremely rich, but there are few studies on the research and protection of genetic diversity of germplasm resources. At present, SSR technology is widely used in the research and development of molecular genetic markers. The primers are designed with tandem sequences of dozens of nucleotides composed of distributed repeat units. The development of SSR-labeled primers must first obtain the sequence information on both sides of the repeat sequence from the species and design the primers before they can be used; However, with the development of taro ...

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Problems solved by technology

my country's taro germplasm resources are extremely rich, but there are few studies on the research and protection of genetic diversity of germplasm resources. At present, SSR technology is widely used in the research and development of molecular genetic markers. The primers are designed by tandem sequences of dozens of nucleotides composed of distributed repeat units. The development of SSR-labeled primers is firstly to obtain the sequence information on both sides of the repeat sequence from the species and design primers, and then it can be used; However, with the development of taro breeding technology, many new taro varieties are constantly emerging, and how to effectively and scientifically classify these varieties, it is not enough to rely on morphological observation alone, and it is necessary to distinguish them at the DNA level. To provide indirect evidence for the classification of taro. Although many SSR primers have been reported, the amplification effect of these primers and the analysis effect on germplasm resources are good or bad. Therefore, it is necessary to further develop the SSR primers of taro to provide new primers for subsequent studies on genetic breeding, genome mapping, gene positioning and identification of species relationship.

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