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Complex microbial inoculant for preventing and treating root-knot ematodes by using biocontrol bacteria and preparation method thereof

A compound bacterial agent and root-knot nematode technology, which is applied in the direction of nematicides, botany equipment and methods, chemicals for biological control, etc., can solve the problems of low control effect and instability, and achieve the purpose of inhibiting root-knot nematodes Nematodes, the effect of suppressing the disease

Inactive Publication Date: 2020-07-10
沃博特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Verticillium chlamydia has a big problem in the control of nematodes, the control effect is low and unstable in the soil environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A compound bacterial agent for controlling root-knot nematodes using biocontrol bacteria

[0032] 1 g of Candida gaoli and 10 g of freeze-dried cells of Verticillium pachyspora were mixed to obtain the composite inoculum.

Embodiment 2

[0034] A compound bacterial agent for controlling root-knot nematodes using biocontrol bacteria

[0035] S01, take the fermentation broth containing 10 ml of Candida gaoli (containing 1.5 g of Candida galeri cells), divide 5 times, 2 ml each time, mix with 10 g of diatomaceous earth, dry at 30°C for 10 min, and add the fermentation broth repeatedly to finish.

[0036] S02, take the fermentation broth containing 10 g of Verticillium pachysporum, 2 ml of fermentation broth each time, mix with the above-mentioned diatomite mixed with Candida, dry at 30° C. for 10 min, and repeatedly add the fermentation broth to completion to obtain the composite Bacterial agent.

Embodiment 3

[0038] A compound bacterial agent for controlling root-knot nematodes using biocontrol bacteria

[0039] 1 g of Candida tropicalis and 10 g of freeze-dried cells of Verticillium pachyspora were mixed to obtain the composite inoculum.

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PUM

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Abstract

The invention discloses a complex microbial inoculant for preventing and treating root-knot nematodes by using biocontrol bacteria and a preparation method thereof. The complex microbial inoculant forpreventing and treating root-knot nematodes by utilizing biocontrol bacteria comprises yeast and verticillium chlamydosporium. When the complex microbial inoculant is applied to tomato planting as abase fertilizer, the incidence of root-knot nematodes can be significantly inhibited.

Description

technical field [0001] The invention relates to the field of fertilizers, in particular to a compound inoculant for preventing and controlling root-knot nematodes by utilizing biocontrol bacteria and a preparation method thereof. Background technique [0002] Root-knot nematodes are widely distributed in tropical and subtropical regions and are obligate parasites of more than 30 common vegetables. Root-knot nematodes affect vegetable growth and reduce vegetable yield. [0003] Verticillium pachyspora is a widely used microbial preparation for inhibiting root-knot nematodes. A major problem with Verticillium pachyspora in controlling nematodes is that the control effect in the soil environment is low and not stable. SUMMARY OF THE INVENTION [0004] The purpose of the present invention is to provide a composite inoculant for controlling root-knot nematodes by biocontrol bacteria. In addition to Verticillium pachyspora, this compound inoculum also contains Candida gaoli. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/32A01N63/30A01N25/08A01P5/00
CPCA01N25/08
Inventor 彭勇彭瑞芳
Owner 沃博特生物科技有限公司
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