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Composite biological preparation for repairing polychlorinated biphenyl polluted soil

A technology of biological agents and polychlorinated biphenyls, applied in the field of microorganisms, can solve the problems of unreasonable compatibility of PCBs degrading bacteria agents and poor degradation efficiency, and achieve the effects of simple preparation methods, soil pollution restoration, and easy methods

Active Publication Date: 2020-06-05
河南国佑生态修复技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the defects of unreasonable compatibility and poor degradation efficiency of PCBs degrading bacterial agents in the prior art, the present invention provides a composite biological agent

Method used

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  • Composite biological preparation for repairing polychlorinated biphenyl polluted soil

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A composite biological preparation prepared from five bacteria including Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus;

[0033] Specifically, the composite biological preparation is prepared according to the following steps:

[0034] 1) Add corn steep liquor 8g, glucose 6g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 0.2g, sodium chloride 0.1g, calcium chloride 0.1g, ferrous sulfate 0.01g, manganese sulfate 0.01g into water, stir Evenly, adjust the pH to 7.0-7.5, and then adjust the volume to 1L to obtain a liquid fermentation medium;

[0035] 2) Activate Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus, and then culture them to the seed solution with a concentration of 30 million cfu / ml, and mix the above five The seed solution was mixed according to the volume ratio of 3:2:2:1:1, and then inoculated into the fermenter containing the li...

Embodiment 2

[0040]A composite biological preparation prepared from five bacteria including Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus;

[0041] Specifically, the composite biological preparation is prepared according to the following steps:

[0042] 1) Add corn steep liquor 8g, glucose 6g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 0.2g, sodium chloride 0.1g, calcium chloride 0.1g, ferrous sulfate 0.01g, manganese sulfate 0.01g into water, stir Evenly, adjust the pH to 7.0-7.5, and then adjust the volume to 1L to obtain a liquid fermentation medium;

[0043] 2) Activate Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus, and then culture them to the seed solution with a concentration of 0.5 billion cfu / ml, and mix the above five The seed solution was mixed according to the volume ratio of 5:3:3:2:2, and then inoculated into the fermenter containing the li...

Embodiment 3

[0048] A composite biological preparation prepared from five bacteria including Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus;

[0049] Specifically, the composite biological preparation is prepared according to the following steps:

[0050] 1) Add corn steep liquor 8g, glucose 6g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 0.2g, sodium chloride 0.1g, calcium chloride 0.1g, ferrous sulfate 0.01g, manganese sulfate 0.01g into water, stir Evenly, adjust the pH to 7.0-7.5, and then adjust the volume to 1L to obtain a liquid fermentation medium;

[0051] 2) Activate Rhodopseudomonas palustris, Citrobacter freundii, Shewanella, Pseudomonas putida and Bacillus pumilus, and then culture them to the seed solution with a concentration of 20 million cfu / ml, and mix the above five The seed solution was mixed according to the volume ratio of 5:3:2:2:1, and then inoculated into a fermenter containing a liquid...

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Abstract

The invention belongs to the technical field of microorganisms, and discloses a compound biological preparation. The compound biological preparation comprises rhodopseudomonas palustris, citrobacter freundii, shewanella, pseudomonas putida and bacillus pumilus. The compound biological preparation disclosed by the invention adopts compatibility of five strains, and the strains promote each other and are matched with a specific carrier, so the degradation capacity of the compound biological preparation to polychlorinated biphenyl is improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a composite biological agent for repairing polychlorinated biphenyl polluted soil. This invention is a divisional application of Chinese invention patent CN2017110195048. Background technique [0002] Polychlorinated biphenyls (polychlorinated biphenyls, PCBs alias: chlorinated biphenyls; PCBs are labeled according to the number of chlorine atoms or the percentage of chlorine. In my country, it is customary to use the number of chlorine-substituted biphenyls (regardless of their substitution positions) The PCB is divided into trichlorobiphenyl (PCB3), tetrachlorobiphenyl (PCB4), pentachlorobiphenyl (PCB5), hexachlorobiphenyl (PCB6), heptachlorobiphenyl (PCB7), octachlorobiphenyl (PCB8 ), nonachlorinated biphenyl (PCB9), decachlorinated biphenyl (PCB10). Polychlorinated biphenyls are one of the 12 persistent organic pollutants first included in the Stockholm Conv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/38C12R1/01C12R1/40C12R1/07
CPCC12N1/20B09C1/10Y02A50/20
Inventor 裘正荣王国平徐丹云
Owner 河南国佑生态修复技术有限公司
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