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Method for extracting catalpol

An extraction method, the technology of catalpol, which is applied in the field of extraction of active ingredients of traditional Chinese medicine, can solve the problems of short service life, high price, and large solvent consumption

Inactive Publication Date: 2020-05-22
南京萌源康德生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former is difficult to improve the production efficiency due to the small load purification amount and many purification steps, and consumes a large amount of solvent; the latter overcomes the shortcomings of the former, but because the macroporous adsorption resin or reversed-phase packing medium is expensive and easily contaminated by impurities, As a result, the service life is very short, regeneration is very difficult, and a large amount of solvent is consumed, and it is difficult to reuse in large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Crush the fresh rehmannia glutinosa by 2-10mm; add the processed rehmannia glutinosa powder into distilled water 1 to 2 times its mass, and then add 2 to 3% of its raw material mass into the prepared methanol preparation solution (with a density of 0.82g / ml~0.89g / ml); the obtained material is placed in an ultrasonic device, and 70% ethanol solution that is 4 times the weight of the raw material is added again, extracted for 3 hours under the conditions of an ultrasonic frequency of 40KHz and a temperature of 60°C, the extract is concentrated under reduced pressure, and the ethanol is recovered to obtain Concentrated solution; the obtained concentrated solution is eluted with 80% ethanol on the macroporous adsorption resin column, and the eluate is collected. The concentration temperature is controlled at ≤50°C, and the vacuum is controlled at -0.05MPa~-0.08MPa. The content of alcohol is 94.56%.

Embodiment 2

[0017] Crush the fresh rehmannia glutinosa by 2-10mm; add the processed rehmannia glutinosa powder into distilled water 1 to 2 times its mass, and then add 2 to 3% of its raw material mass into the prepared methanol preparation solution (with a density of 0.82g / ml~0.89g / ml); the obtained material is placed in an ultrasonic device, and 85% ethanol solution of 5 times the weight of the raw material is added again, extracted for 3.5 hours under the conditions of an ultrasonic frequency of 40KHz and a temperature of 65°C, the extract is concentrated under reduced pressure, and the ethanol is recovered. Obtain a concentrated solution; elute the obtained concentrated solution with 80% ethanol on a macroporous adsorption resin column, collect the eluate, control the concentration temperature at ≤ 50°C, and control the vacuum at -0.05MPa~-0.08MPa, and detect through UV. The content of catalpol is 96.46%.

Embodiment 3

[0019] Crush the fresh rehmannia glutinosa by 2-10mm; add the processed rehmannia glutinosa powder into distilled water 1 to 2 times its mass, and then add 2 to 3% of its raw material mass into the prepared methanol preparation solution (with a density of 0.82g / ml~0.89g / ml); the obtained material is placed in an ultrasonic device, and 95% ethanol solution of 6 times the weight of the raw material is added again, extracted for 4 hours under the conditions of an ultrasonic frequency of 40KHz and a temperature of 70°C, the extract is concentrated under reduced pressure, and ethanol is recovered to obtain Concentrated solution; the obtained concentrated solution is eluted with 80% ethanol on the macroporous adsorption resin column, and the eluate is collected. The concentration temperature is controlled at ≤50°C, and the vacuum is controlled at -0.05MPa~-0.08MPa. The content of alcohol is 93.96%.

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Abstract

The invention relates to a method for extracting catalpol and in particular relates to a method for ultrasonic extraction on catalpol. The method comprises the following treatment steps: crushing fresh rehmannia roots into 2-10mm; adding distilled water which is 1-2 times of the mass of the treated rehmannia root powder into the treated rehmannia root powder, and further adding a prepared methanolblending liquid (the density is 0.82-0.89g / ml) which is 2-3% by weight of the raw materials; putting the obtained material into an ultrasonic device, further adding a 70-95% ethanol solution which is4-6 times of the weight of the raw materials, performing extraction for 3-4 hours under conditions that the ultrasonic frequency is 40KHz and the temperature is 60-70 DEG C, performing vacuum concentration on the extract, and recycling ethanol so as to obtain a concentrated solution; eluting the obtained concentrated solution by using 80% ethanol on a macroreticular resin column, collecting eluant, controlling the concentration temperature to be less than or equal to 50 DEG C, controlling the vacuum degree to minus 0.05MPa to minus 0.08MPa, and performing drying, so as to obtain catalpol. Themethod provided by the invention is simple and feasible, is high in content of a purification substance, namely the catalpol, is good in reproducibility, and in addition, a resin can be repeatedly used.

Description

Technical field: [0001] The invention relates to a method for extracting catalpol, which belongs to the field of extracting effective components of traditional Chinese medicines. Background technique: [0002] Catalpol has a wide range of pharmacological effects and is expected to become a new therapeutic drug. Catalpol-containing plant resources in my country are quite rich, especially plants with high content, such as Rehmannia glutinosa, which is a large number of commonly used medicinal materials in my country, which provides a resource guarantee for the development of new catalpol drugs. For this reason, the chemical transformation and pharmacodynamics of catalpol and its derivatives should be studied together, in order to clarify the internal mechanism of the change of medicinal properties of rehmannia glutinosa through processing, and open up a feasible way, the research on catalpol and its degradation should be strengthened. The active products that may be generated...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
CPCC07H1/08C07H17/04
Inventor 王俊
Owner 南京萌源康德生物技术有限公司
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