A method for directional regulation of endogenous functional microorganisms in oil reservoirs
A technology of functional microorganisms and microorganisms, which is applied in chemical instruments and methods, earth-moving drilling and drilling compositions, etc., can solve the problems of directional and efficient activation of functional microorganisms, poor targeting of functional microorganisms, etc., and achieves good field test results. The effect of reducing the dosage and the method is reasonable
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Embodiment 1
[0038] Test block G of an oil production plant in Shengli Oilfield 12 Overview: Reservoir temperature is 70°C, reservoir pressure is 12.3MPa, reservoir thickness is 6.5m, permeability is 800×10 -3 μm 2 , porosity 28%, crude oil viscosity 600mPa·s, comprehensive water cut 96.5%, recovery degree 15.8%, formation water salinity 11500mg / L, pore volume 2.0×10 5 m 3 , geological reserves 7.2×10 4 t. Utilize the method of the present invention to carry out microbial flooding to enhance oil recovery in this block, and concrete steps are as follows:
[0039] (1) Screening of test reservoirs
[0040] After sampling, testing and analysis, the test block G 12 There are emulsifier-producing microorganisms Brevibacterium and sulfate-reducing bacteria Desulfovibrio in formation water. Meet the screening criteria of the reservoir of the present invention.
[0041] (2) Phage isolation and amplification of sulfate-reducing bacteria
[0042] Sulfate-reducing bacteria phage isolation: ta...
Embodiment 2
[0064] Test block G of an oil production plant in Shengli Oilfield 18 Overview: Reservoir temperature is 72°C, reservoir pressure is 10.3MPa, reservoir thickness is 10.8m, permeability is 1200×10 -3 μm 2 , porosity 32.5%, crude oil viscosity 1523mPa·s, comprehensive water cut 97.2%, recovery degree 15.2%, formation water salinity 25682mg / L, pore volume 5.0×10 5 m 3 , geological reserves 1.2×10 5 t. Utilize the method of the present invention to carry out microbial flooding to enhance oil recovery in this block, and concrete steps are as follows:
[0065] (1) Screening of test reservoirs
[0066] After sampling, testing and analysis, the test block G 18 Biosurfactant-producing microorganisms Bacillus and sulfate-reducing bacteria Desulfococcus are present in formation water. Meet the screening criteria of the reservoir of the present invention.
[0067] (2) Phage isolation and amplification of sulfate-reducing bacteria
[0068] Sulfate-reducing bacteria phage isolation...
Embodiment 3
[0090] Test block F of an oil production plant in Shengli Oilfield 21 Overview: Reservoir temperature is 85°C, reservoir pressure is 13.2MPa, reservoir thickness is 15.2m, permeability is 1000×10 -3 μm 2 , porosity 31.2%, crude oil viscosity 1152mPa·s, comprehensive water cut 97.8%, recovery degree 23.5%, formation water salinity 22785mg / L, pore volume 8.0×10 5 m 3 , geological reserves 2.1×10 5 t. Utilize the method of the present invention to carry out microbial flooding to enhance oil recovery in this block, and concrete steps are as follows:
[0091] (1) Screening of test reservoirs
[0092] After sampling, detection and analysis, the test block F 21 There are emulsifier-producing microorganisms Rhodococcus and sulfate-reducing bacteria Desulfobacter in formation water. Meet the screening criteria of the reservoir of the present invention.
[0093] (2) Phage isolation and amplification of sulfate-reducing bacteria
[0094] Sulfate-reducing bacteria phage isolation...
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