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Bacillus amyloliquefaciens, bacteria agent and its application and method for degrading formaldehyde

A technology for dissolving starch spores and bacilli, applied in the field of microorganisms, can solve the problems of formaldehyde tolerance and poor degradation effect, achieve excellent degradation effect, reduce investment and operating costs, and reduce interference.

Active Publication Date: 2021-08-10
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of poor formaldehyde tolerance and degradation effect of formaldehyde-degrading bacteria in the prior art, and to provide a microbial strain that can tolerate high-concentration formaldehyde and can efficiently degrade formaldehyde, so as to realize the rapid biological method. The purpose of efficiently degrading formaldehyde

Method used

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  • Bacillus amyloliquefaciens, bacteria agent and its application and method for degrading formaldehyde
  • Bacillus amyloliquefaciens, bacteria agent and its application and method for degrading formaldehyde
  • Bacillus amyloliquefaciens, bacteria agent and its application and method for degrading formaldehyde

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Embodiment approach

[0057] According to a specific embodiment of the present invention, the inorganic salt comprises sodium chloride, potassium dihydrogen phosphate, disodium hydrogen phosphate, ammonium chloride, magnesium sulfate, calcium chloride and ferrous sulfate.

[0058] Wherein, the present invention has no special limitation on the amount of the inorganic salt added, which can be determined according to the type and content of the inorganic salt in the formaldehyde-containing environment. For example, take NH 4 Cl 0.5-4g·L -1 , NaCl 4-8g·L -1 , KH 2 PO 4 0.2-0.8g·L -1 , Na 2 HPO 4 0.2-0.8g·L -1 , CaCl 2 0.01-0.05g·L -1 , MgSO 4 0.1-0.5g·L -1 For example, based on each kilogram of the environment containing formaldehyde and / or the environment containing formaldehyde, the amount of the above-mentioned inorganic salt mixture can be 1-3ml / day.

[0059] In the present invention, when the formaldehyde-containing environment is soil, in order to further promote the degradation e...

preparation example

[0081] This preparation example is used to prepare the activated seed solution used in the following examples

[0082] The LB medium was sterilized at 121°C for 20 min and cooled to room temperature. Take Bacillus amyloliquefaciens strains from the refrigerator at -80°C, inoculate them in the above-mentioned sterilized LB medium, and cultivate them at 170rpm and 30°C for 12 hours to obtain activated seed liquid. The number of viable bacteria was determined to be 7.88× 10 8 cfu / mL.

Embodiment 1

[0084] This embodiment is used to illustrate the impact of culture conditions on the ability of bacterial strains to degrade formaldehyde according to the present invention

[0085] Get the activated seed solution after activation and inoculate it into carbon-free medium with an initial concentration of formaldehyde of 20 mg / L according to different inoculum amounts (8%, 10%, 12%, 15%, 18%, 20%) respectively. Three parallels were set up for each gradient, and the blank culture medium was used as the control. The shaker flask was cultured in a constant temperature shaker with a rotation speed of 170r / min and a temperature of 30°C. Samples were taken at 2h, 4h, 6h and 14h to determine the formaldehyde concentration, OD600 and pH in the medium.

[0086] Take the bacterial solution cultivated to the logarithmic phase and add it to the sterilized and cooled medium with an initial formaldehyde concentration of 300 mg / L at the optimal inoculum amount, and select different culture tem...

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Abstract

The invention relates to the field of microorganisms, and specifically discloses a strain of Bacillus amyloliquefaciens, a bacterial agent containing the Bacillus amyloliquefaciens and an application thereof, wherein the preservation number of the Bacillus amyloliquefaciens is CGMCC No. 18396. Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) of the present invention can survive and grow in an environment containing formaldehyde, and can also survive and grow in an environment of oil fume condensate, and the bacterial strain can also tolerate high concentrations of formaldehyde and oil fume condensate . The present invention provides the core bacterial strain for the biodegradation of formaldehyde in cooking fumes, thereby reducing the investment and operating costs of degrading formaldehyde. It has the advantages of in-situ pollutant degradation and removal, easy operation, less interference, and less secondary pollution. It is suitable for large-scale Large-scale fermentation production and long-term use.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a strain of Bacillus amyloliquefaciens and a bacterium containing the bacillus, their application in degrading formaldehyde, treating oil fume condensate and degrading grease, and a method for degrading formaldehyde. Background technique [0002] Formaldehyde is a colorless, highly irritating, toxic gas. Formaldehyde ranks second on my country's priority control list, and has been identified as carcinogenic and teratogenic substances by the World Health Organization, with strong carcinogenic and cancer-promoting effects. The source of formaldehyde involves many industries, such as decoration and building materials, and the chemical industry. In addition, with the improvement of environmental quality and the systematic research on the emission characteristics of pollution sources, it is found that a large amount of formaldehyde will also be emitted in oil fume. At present, no effective tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/07
CPCB01D53/72B01D53/84B01D2251/95C12N1/20C12N1/205C12R2001/07Y02A50/20
Inventor 史纯珍韩茜姚志良申现宝姚小龙
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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