Molecular markers closely linked with major QTL of wheat plant height and application of molecular markers

A technology of molecular markers and wheat, applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of no patent report on the target site, so as to promote transfer application, improve selection efficiency and quality, The effect of shortening the time

Active Publication Date: 2020-04-10
LUDONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there are few reports on the main QTL for wheat plant height at the 5A locus, and there is no relevant patent report on the target locus

Method used

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  • Molecular markers closely linked with major QTL of wheat plant height and application of molecular markers
  • Molecular markers closely linked with major QTL of wheat plant height and application of molecular markers
  • Molecular markers closely linked with major QTL of wheat plant height and application of molecular markers

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Embodiment Construction

[0037] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0038] The wheat variety Kenong 9204 is an approved variety, which was approved by the Hebei Provincial Crop Variety Approval Committee in 2002, and passed the national variety approval in 2003. The variety approval number is National Examination Mai 2003037.

[0039] Wheat variety Jing 411 is an approved variety. It passed the Beijing variety approval in 1991, and passed the Tianjin, Shanxi Province and national variety approval in 1992. It can be obtained from the National Crop Germplasm Resource Bank, and the national uniform number is ZM020984.

[0040] In this specific embodiment, 2×Taq PCR StarMix is ​​premixed high-purity TaqDNA polymerase containing optimized concentration, dNTPs, Mg 2+ The ready-to-use 2-fold concentration PCR solution of components such as reaction buffer and optimizer, the product brand is TIANGEN, provided by ...

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Abstract

The invention discloses molecular markers closely linked with the major QTL of wheat plant height and application of the molecular markers. The number of the molecular markers related to the wheat plant height character is two; one molecular marker is a DNA fragment obtained by taking the genome DNA of Beijing 411 wheat as a template and carrying out PCR amplification with a primer pair I shown inSEQ ID NO: 1 and SEQ ID NO: 2, and is marked as qPH-5A-ID2; and the other molecular marker is a DNA fragment which is obtained by taking the genome DNA of Beijing 411 wheat as the template and carrying out PCR amplification with a primer pair II as shown in SEQ ID NO: 3 and SEQ ID NO: 4, and is marked as qPH-5A-ID3. The molecular markers qPH-5A-ID2 and qPH-5A-ID3 provided by the invention are closely linked with qPH-5A, so transfer and application of the locus in promotion of varieties can be promoted, and pyramiding breeding of the locus and other yield-related loci is also facilitated.

Description

technical field [0001] The invention relates to a molecular marker and its application, in particular to a molecular marker closely linked with wheat plant height main effect QTL and its application, and belongs to the technical field of crop seed selection and cultivation. Background technique [0002] Wheat is the second largest food crop in the world, providing about 20% of food calories and protein for humans. With the world population expected to grow to 9.6 billion by 2050, wheat production will need to increase by 1.6% per year to meet future demand. Plant height is an important constituent factor of wheat plant type, which is closely related to photosynthetic efficiency and yield. Therefore, analyzing the molecular genetic basis of yield-related traits, mining alleles of superior plant height and using them in breeding will help the cultivation of new high-yield varieties of wheat and ensure food security and sustainable agricultural development in China. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2565/125
Inventor 吴永振孙晗荀海兰崔法赵春华秦冉徐彗渊韦美祥张尚辉马成学黄晓倩张才军
Owner LUDONG UNIVERSITY
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