Industrialized hybrid seed production for bupleurum

A technology for hybrid seed production and Bupleurum, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of species degradation, mixed germplasm, hinder the industrialization promotion of cultivated Bupleurum, and avoid emasculation. Work, the effect of significant superparent advantage

Inactive Publication Date: 2019-12-20
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, all new varieties of Bupleurum are systematically selected, and in production, Yaonong prefers to save their own seeds
However, Bupleurum is a cross-pollinated plant, and irregular breeding can easily lead to serious germplasm mixing and species degradation, which seriously hinders the industrialization of cultivated Bupleurum.
At the same time, due to the long-term systematic breeding, the breeding of Bupleurum bupleurum for high yield, high efficiency and high adaptability is also facing a bottleneck

Method used

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  • Industrialized hybrid seed production for bupleurum

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Effect test

Embodiment 1

[0016] This embodiment specifically provides an industrialized Bupleurum hybrid seed production method, comprising the following steps:

[0017] 1) For seedling raising and cultivation, Bupleurum germination, soil preparation and sowing methods refer to the previous patent of the research group (patent name: a high-yield cultivation method of Bupleurum, patent application number: 201910190238.8). The seedlings are thinned at the two-leaf stage after emergence, and the seedling density is 30,000 seedlings per mu.

[0018] Specifically include the following steps:

[0019] Bupleurum germination: From March to April every year (before Qingming Festival), take the dried Bupleurum seeds and soak them in water for 30 minutes, and remove the floating grains and impurities while stirring the seeds in the water. Seeds were placed in 220mg / L gibberellin solution for 24 hours under the environment of 25 degrees. Mix the soaked and dried seeds with the peat soil at a ratio of 1:10 by ma...

Embodiment 2

[0027] Chuanbei Chai No. 1 (CBC 1), Rongxian Bupleurum (BM) and their F1 hybrids (CBC 1 as the female line and BM as the male parent) were used, and they were sown on the farm of Southwest University of Science and Technology in March 2018. The field design was sequential sowing, and 3 biological replicates were set up. The planting area of ​​each genotype is 0.5 mu, the planting density is 60,000 plants / mu, conventional fertilizer and water management and pest control. After the harvest in November of that year, it was found that the production-related indicators were

[0028] The ratio of root dry weight to fresh weight (RDW / RFW) of Chuanbei Bupleurum 1 (CBC 1) group was significantly higher than that of the control group (P<0.01). RL (root length), RT (root diameter), RFW (root fresh weight), RDW (root dry weight) of Rongxian Bupleurum (BM) compared with F1 generation and Chuanbei Bupleurum 1 (CBC 1) generation , RDW / RFW (reduced dry ratio: ratio of root dry weight to fre...

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Abstract

The invention discloses an industrialized hybrid seed production for bupleurum. The method comprises the steps that a bupleurum male parent resource is planted, female parent is seeded in front and back rows close to the rows of the male parent after the second week and the fourth week, when the male parent and the female parent enter the full blooming stage at the same time, the female parent terminal inflorescences obtained after pollen flying and all young and tender inflorescences except primary inflorescences are removed, the plants with the primary inflorescences flowering in advance orlag of flowering are pulled out, 2-7 days after pollen flying of female parent inflorescences is finished, male parent branches are slightly shaken to pollinate female parent pistils, after grains aremature, the grains become yellow from green and finally become dark brown, and after harvesting and drying, a lot of hybrid F1 seeds are obtained. By means of the method, a lot of castration work canbe prevented, and the F1 plants obtained by using the method have significant heterobeltiosis in the aspects of yield and effective constituent content.

Description

technical field [0001] The invention belongs to the technical field of Bupleurum seed production, and in particular relates to an industrialized Bupleurum hybrid seed production method. Background technique [0002] Radix Bupleuri is a common bulk medicinal material used in Asian countries such as my country, Japan and Korea. With the sharp decline in resource reserves of wild Bupleurum, artificial cultivation of Bupleurum appears to be particularly important. At present, 4 new varieties of Bupleurum chinense DC, Zhongchai 1-3 and Chuanbeichai 1, and 2 new varieties of Bupleurum chinense DC, Zhonghongchai 1 and Chuanhongchai 1, have been bred through selective breeding (B. scorzonerifolium Willd). Compared with local cultivated species and wild species, these varieties show higher variety consistency, yield and efficacy stability, and solve the problems of slow germination and irregular emergence of Bupleurum seeds. However, all new varieties of Bupleurum are systematical...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 余马侯大斌陈华唐志康舒晓燕黄晶
Owner SOUTHWEAT UNIV OF SCI & TECH
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