The Method of Substituting Phellinus Phellinus for Stereo-Culture with Ding-shaped Deep Mouth Double Membrane
A technology of three-dimensional culture and Phellinus, which is applied in botany equipment and methods, cultivation, mushroom cultivation, etc., can solve the problems of low utilization rate of a single square of the cultivation site, difficulty in Phellinus, easy to form bacterial skin, etc., and achieve single square High utilization rate, shortened cultivation period, plump and beautiful appearance
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Embodiment 1
[0038] (1) Preparation of Phellinus japonica culture medium:
[0039] The ingredients are formulated according to the following mass fractions: 85% sawdust, 10% wheat bran, 4% soybean powder and 1% gypsum. Adjust the pH value to 7-7.5 with NaOH or dilute hydrochloric acid to obtain a uniformly dispersed Phellinus medium; sawdust is granular oak wood chips of 0.5-0.8 cm.
[0040] (2) the uniformly dispersed Phellinus substratum is packed into the bacteria bag with an automatic bagging machine, and the weight of filling the culture medium in each bacteria bag is 1kg; the size of the bacteria bag is 16.2 × 36, and the weight of the bacteria bag is 7.5g / indivual.
[0041] (3) Sterilization:
[0042] The bacteria bag obtained in step (2) is sterilized by autoclaving, and the autoclaving is to keep the temperature at 121-126°C, the pressure at 0.05-0.1Mpa, and sterilize for 4.5h;
[0043] (4) Inoculation:
[0044] After sterilization, when the material temperature of the fungus...
Embodiment 2
[0054] (1) the preparation of Phellinus japonica culture medium is the same as embodiment 1;
[0055] The ingredients are formulated according to the following mass fractions: 85% sawdust, 10% rice bran, 4% soybean powder and 1% gypsum. Dry mix the above mixture evenly, then add water and stir evenly to ensure that the moisture content is 58-60%; use 10% NaOH Or dilute hydrochloric acid to adjust the pH value to 7-7.5 to obtain a uniformly dispersed Phellinus medium; sawdust is 0.5-0.8cm granular oak wood chips.
[0056] (2) Pack the uniformly dispersed Phellinus culture medium into the bacteria bag with an automatic bagging machine, and the weight of the culture medium in each bacteria bag is 1.5kg; the size of the bacteria bag is 16.2×38, and the weight of the bacteria bag is 7.5g / indivual.
[0057] (3) Sterilization:
[0058] The bacteria bag obtained in step (2) is sterilized by autoclaving, and the autoclaving is to keep the temperature at 121-126°C, the pressure at 0....
Embodiment 3
[0070] (1) Preparation of Phellinus japonica culture medium:
[0071] The ingredients are formulated according to the following mass fractions: 85% sawdust, 5% wheat bran, 5% rice bran, 4% soybean flour and 1% gypsum. Dry mix the above mixture evenly, then add water and stir evenly to ensure that the moisture content is 58-60%. ; use 10% NaOH or dilute hydrochloric acid to adjust the pH value to 7-7.5 to obtain a uniformly dispersed Phellinus medium; sawdust is granular oak wood chips of 0.5-0.8 cm.
[0072] (2) Pack the evenly dispersed Phellinus culture medium into the bacteria bag with an automatic bagging machine, and the weight of the culture medium in each bacteria bag is 1.2kg; the size of the bacteria bag is 16.2×38, and the weight of the bacteria bag is 7.5g / indivual.
[0073] (3) Sterilization:
[0074] The bacterial bag obtained in step (2) is sterilized under normal pressure, and the normal pressure sterilization is to keep the temperature at 102° C., and steril...
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