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Absolute quantification analysis method of ceramide

A ceramide, quantitative analysis technology, applied in the field of absolute quantitative analysis of ceramide, can solve the problem that the analysis method cannot guarantee specificity and quantitative performance, achieve low sample consumption, ensure sensitivity and specificity, and ensure reproducibility and stability effects

Inactive Publication Date: 2019-12-06
天津云检医学检验所有限公司 +1
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  • Abstract
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Problems solved by technology

[0005] The technical problem to be solved by the technical solution of the present invention is that the existing analysis method cannot effectively quantify the molecular number of ceramide with a small amount of serum under the premise of ensuring the specificity and quantitative performance

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  • Absolute quantification analysis method of ceramide
  • Absolute quantification analysis method of ceramide
  • Absolute quantification analysis method of ceramide

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Embodiment Construction

[0041] In order to enable those skilled in the art to better understand the technical solutions in the application, the present invention will be further described below in conjunction with the following examples. Obviously, the described embodiments are only a part of the embodiments of the application, rather than all Example. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts shall fall within the scope of protection of this application. The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0042] The embodiment of the present invention takes the analysis of ceramide in serum as an example to describe the absolute quantitative analysis method of the technical solution of the present invention in detail. Of course, in addition to detecting serum, the detection method of the technical solution of the present invent...

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Abstract

The technical solution of the present invention discloses an absolute quantification analysis method of ceramide. After a to-be-tested sample, a working reagent and a solvent are uniformly mixed, a mixture is stratified to form an upper-layer to-be-tested sample and a lower-layer to-be-tested sample, the upper-layer to-be-tested sample and a calibration reagent are detected by using a high performance liquid chromatography-tandem mass spectrum system, to obtain a mass spectrum detection result of the calibration reagent, calibration is performed according to mass spectrum detection results ofat least two calibration reagents, and absolute quantification analysis is performed on the ceramide in the to-be-tested sample according to a calibration result and a mass spectrum detection result of the upper-layer to-be-tested sample. According to the analysis method provided in the technical solution of the present invention, molecular number absolute quantification on ceramide can be effectively performed by using only a small quantity of serum.

Description

technical field [0001] The invention belongs to the field of quantitative analysis of compounds, in particular to an absolute quantitative analysis method of ceramide. Background technique [0002] Blood is composed of cells and body fluid components. The cell components include red blood cells, white blood cells, and platelets. The body fluid is plasma (serum), which contains various proteins with special functions and a large number of small molecule metabolites. Blood circulates back and forth in the body, and is interdependent and interacting with various tissues of the human body, which can reflect the metabolic changes of physiological and pathological conditions in the body. Through the detection and analysis of these metabolites, understanding the subtle differences, characteristics and accompanying phenomena between them can provide We provide important clues or experimental evidence. Amino acids, acylcarnitines and fatty acids are important metabolites in the bloo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/7266
Inventor 凌雪峰
Owner 天津云检医学检验所有限公司
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