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Preparation method and application for electrochemiluminescence sensor based on protein activity protection

A protein activity and electrochemical technology, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis of materials through chemical reactions, can solve problems such as difficult electrode surfaces and biological toxicity, and achieve high sensitivity and reaction. Fast, wide-range effects

Active Publication Date: 2019-11-12
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses Eu3/Ga_dopant (Eu: Europium), which helps create an electronically active substances that emit visible lights when exposed to certain chemicals or stimuli like UV rays. These materials have unique properties such as large surface areas with holes inside them called cages made up by atoms connected through oxygen bridges between different parts - these holes allow charges from outside molecules to enter into their lattices without being blocked off – allowing charged particles within it to move freely around while still emitting fluorescent radiation. By adding another atom next to each hole, they become more efficient than traditional organic dyes used previously due to its larger size compared to other commonly known luminescers. Overall, this innovation makes possible applications where detectable labels may help diagnose diseases faster and easier.

Problems solved by technology

The technical problem addressed in this patented patent relates to improving the accuracy and reliability of diagnosing diseases like sepsis through identifying changes associated with elevated levels of certain substances called endogenesis markers (ECFAs) released from damaged tissues during surgery. Current systems require complicated procedures involving multiple chemical agents, leading to decreased effectiveness over time due to their poor selectivity towards different EFCA types. This results in increased risk of complications related to blood clots and cardiovascular events.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1 europium-doped gadolinium phosphate

[0038] (1) Preparation of phenolic resin template spheres

[0039] Add 0.1806 g of 3-aminophenol and 93 µL of 25% ammonia water into 50.4 mL of deionized water, and stir at 30°C for 10 min to form a clear solution. After adding 123 µL of formaldehyde solution, the solution turned white after 30 s. After stirring continuously for another 4 h, the resulting mixture was transferred into a 100 mL Teflon-lined stainless steel autoclave and kept at 100 ºC for 24 h. Finally, the sample was centrifuged and purified by washing twice with deionized water and alcohol, and finally dried in air;

[0040] (2) Preparation of europium-doped gadolinium phosphate

[0041] First, the precursors were prepared: 3 g of urea, 1 mmol of gadolinium nitrate aqueous solution, 0.05 mmol of europium nitrate aqueous solution and 0.2 g of the resin balls synthesized above were added to 25 mL of deionized water. Stir for 30 min,...

Embodiment 2

[0042] Example 2 Preparation of palladium-functionalized cuprous oxide-labeled procalcitonin capture antibody incubation solution

[0043] (1) Preparation of palladium nanocrystals

[0044] First, 0.048 g of cetyltrimethylammonium chloride, 9.125 mL of deionized water, and 0.7 mL of 10 mM chloropalladium acid solution were injected into the vial, which was stored in a 35ºC water bath. Subsequently, 500 µL of 1 mM potassium bromide solution and 50 µL of 1 mM potassium iodide solution were added and mixed. After 10 min, inject 1.2 mL of 0.05 M ascorbic acid, keep the water bath for 30 min, and centrifuge at 7500 rpm for 10 min twice. Final product in 400 µL of deionized water;

[0045] (2) Preparation of palladium-functionalized cuprous oxide

[0046] Dissolve 0.087 g sodium lauryl sulfate in deionized water, add 0.07 mL of 0.1 M copper chloride solution and 0.08 mL of palladium nanocrystal solution prepared in the previous step, and inject 0.25 mL of 1.0 M sodium hydroxide s...

Embodiment 3

[0049] Example 3 Preparation of palladium-functionalized cuprous oxide-labeled procalcitonin capture antibody incubation solution

[0050] (1) Preparation of palladium nanocrystals

[0051] First, 0.0100 g of cetyltrimethylammonium chloride, 9.125 mL of deionized water, and 0.2 mL of 10 mM chloropalladium acid solution were injected into the vials, which were kept in a 35ºC water bath environment. Subsequently, 300 µL of 1 mM potassium bromide solution and 50 µL of 1 mM potassium iodide solution were added and mixed. After 10 min, 0.5 mL of 0.05 M ascorbic acid was injected. Keep the water bath for 30 min and centrifuge at 7500 rpm for 10 min twice. Final product in 400 µL of deionized water;

[0052] (2) Preparation of palladium-functionalized cuprous oxide

[0053] Dissolve 0.020 g sodium lauryl sulfate in deionized water, add 0.03 mL of 0.1 M copper chloride solution and 0.02 mL of palladium nanocrystal solution prepared in the previous step, and inject 0.25 mL of 1.0 M...

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Abstract

The invention relates to a preparation method and application for an electrochemiluminescence sensor based on protein activity protection and belongs to the technical field of electrochemiluminescencedetection. An efficient electrochemiluminescence behavior capable of being achieved by europium-doped gadolinium phosphate through excitation under low potential is developed and is verified for thefirst time. The fixing problem of a luminescent material on an electrode is solved. The effective preservation problem of antigen-antibody activity is also solved. Concentrations are detected according to the fact that intensities of electrochemiluminescence signals responding to procalcitonins of different concentrations are different. Through F-test and T-test, accuracy and precision of the method are shown. Test results are smaller than a theoretical value. The method is accurate and reliable.

Description

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Claims

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Application Information

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Owner UNIV OF JINAN
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