A kind of preparation method of frozen section of penaeus vannamei gonad tissue

A technology of frozen section and production method, which is applied in the preparation, sampling, and instrumentation of test samples, which can solve the problems of long time, low efficiency, and poor slicing effect, and achieve good slicing effect, save time, and high slicing efficiency effect

Active Publication Date: 2021-07-30
ZHEJIANG MARICULTURE RES INST
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The traditional frozen section technology is mainly used to cut fresh tissue, but after the sample is fixed by formaldehyde, paraffin section technology is used for sectioning, which takes a lot of time and is not efficient. Although the efficiency of section staining is high, the section effect is often not good. Therefore, the present invention has developed a method for staining sample sections after formaldehyde fixation, which not only has good efficiency, but also has good staining effect.

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  • A kind of preparation method of frozen section of penaeus vannamei gonad tissue
  • A kind of preparation method of frozen section of penaeus vannamei gonad tissue

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0017] A method for making frozen section of penaeus vannamei gonad tissue, the steps are as follows:

[0018] (1) Dehydration treatment of tissue after formaldehyde fixation: take the ovary tissue of Penaeus vannamei and fix it in 10% formaldehyde solution for later use, then cut the ovary tissue with a length of 0.5-1.0 cm with a scalpel blade, soak it in 20% sucrose solution and OCT Frozen embedding agent volume 1:1 liquid, soaking time at least 16 hours;

[0019] (2) Tissue embedding: Take out the ovarian tissue soaked in step (1), put it into a cylindrical hose model containing OCT frozen embedding agent, use tweezers to pick up the model and freeze it in liquid nitrogen for 15-20 s, then put it into the cooled cryostat, remove the model and get the sample, fix the sample on the pre-cooled sample holder with OCT frozen embedding agent, put it on t...

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Abstract

The invention discloses a method for making frozen section of gonad tissue of Penaeus vannamei. The steps are as follows: dehydration treatment of the tissue after formaldehyde fixation, tissue embedding, frozen section, staining and sealing. The method provided by the invention is different from the frozen section technique of fresh tissue for the frozen section technique of the gonad tissue of Penaeus vannamei after formaldehyde fixation. Compared with the traditional paraffin sectioning method, it has higher sectioning efficiency and saves time. Compared with the general frozen section method, it has a better section effect and is conducive to staining observation.

Description

technical field [0001] The invention relates to a method for making a frozen section, more specifically, it relates to a method for making a frozen section of the gonad tissue of Penaeus vannamei. Background technique [0002] Litopenaeus vannamei, also known as Penaeus vannamei, belongs to the order Deeapoda, the family Penaeidae, and the genus Litopenaeus. It is native to the Pacific coast from Peru to Mexico in Central and South America. It is the leading species in the industry and was introduced into China in 1988 as an alternative species of Chinese prawn (Fenneropenaeus chinensis). Because Litopenaeus vannamei has the advantages of fast growth, short breeding cycle and strong breeding adaptability, the breeding area has expanded rapidly after 2000. It is already one of the pillar industries of aquaculture in China. [0003] The traditional frozen section technology is mainly used to cut fresh tissue, but after the sample is fixed by formaldehyde, paraffin section tec...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/36G01N1/42G01N1/06
CPCG01N1/06G01N1/2813G01N1/286G01N1/30G01N1/36G01N1/42
Inventor 张敏王瑶华李方冀德伟胡利华闫茂仓柴雪良
Owner ZHEJIANG MARICULTURE RES INST
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