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A method for increasing the weight of silk glands of silkworm

A technology of weight and silk, applied in the field of increasing the weight of silk glands in silkworm, can solve problems such as lack of research, and achieve the effect of improving economic traits of silkworm

Active Publication Date: 2020-07-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bombyx mori lipokinetic hormone is one of silkworm neuropeptides, its main function is to regulate the dynamic balance and distribution of energy in silkworms, but there is a lack of research on silkworm neuropeptides regulating the growth of silk glands through energy distribution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] According to the sequence pQLTFTSGWGa, an amino acid protected with an α-amino group by a 9-fluorenylmethoxycarbonyl (Fmoc) group is covalently linked to an insoluble solid-phase support (para-alkoxybenzyl alcohol arm) benzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to take off the Fmoc protecting group on the α-amino group, wash the amino acid--arm--resin with the TFA solution of 90% mass fraction, the next The pre-activated α-amino protected amino acids are connected by a coupling reaction; after the reaction is completed, wash with 90% mass fraction of TFA solution, and then repeat the deprotection and coupling reactions until all amino acids are sequenced from the C-terminus ( carboxyl terminal) to the N terminal (amino terminal) to obtain the target polypeptide; use hydrogen fluoride (HF) to hydrolyze the ester bond between the peptide chain and the solid phase carrier to obtain the silkworm adipokinin A polypeptide synthesis solution...

Embodiment 2

[0035] According to the sequence pQLTFTSGWGa, an amino acid protected with an α-amino group by a 9-fluorenylmethoxycarbonyl (Fmoc) group is covalently linked to an insoluble solid-phase support (para-alkoxybenzyl alcohol arm) benzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to take off the Fmoc protecting group on the α-amino group, wash the amino acid--arm--resin with the TFA solution of 90% mass fraction, the next The pre-activated α-amino protected amino acids are connected by a coupling reaction; after the reaction is completed, wash with 90% mass fraction of TFA solution, and then repeat the deprotection and coupling reactions until all amino acids are sequenced from the C-terminus ( carboxyl terminal) to the N terminal (amino terminal) to obtain the target polypeptide; use hydrogen fluoride (HF) to hydrolyze the ester bond between the peptide chain and the solid phase carrier to obtain the silkworm adipokinin A polypeptide synthesis liquid. ...

Embodiment 3

[0040] According to the sequence pQLTFTSGWGa, an amino acid protected with an α-amino group by a 9-fluorenylmethoxycarbonyl (Fmoc) group is covalently linked to an insoluble solid-phase support (para-alkoxybenzyl alcohol arm) benzyl alcohol type resin); use 90% mass fraction of trifluoroacetic acid (TFA) to take off the Fmoc protecting group on the α-amino group, wash the amino acid--arm--resin with the TFA solution of 90% mass fraction, the next The pre-activated α-amino protected amino acids are connected by a coupling reaction; after the reaction is completed, wash with 90% mass fraction of TFA solution, and then repeat the deprotection and coupling reactions until all amino acids are sequenced from the C-terminus ( carboxyl terminal) to the N terminal (amino terminal) to obtain the target polypeptide; use hydrogen fluoride (HF) to hydrolyze the ester bond between the peptide chain and the solid phase carrier to obtain the silkworm adipokinin A polypeptide synthesis liquid. ...

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Abstract

The invention discloses a method for increasing the weight of silk gland of bombyx mori. According to the sequence pQLTFTSGWGa, bombyx mori adipokinetic hormone A polypeptide synthesis is carried out from a C terminal (carboxyl terminal) to an N terminal (amino terminal); the activity of synthesized bombyx mori adipokinetic hormone A polypeptides is detected by adopting a luciferase reporter gene method; a bombyx mori adipokinetic hormone A active polypeptide solution is degermed by a filtering membrane, and injected into the abdomen of the 5-day bombyx mori in the second day to the third day, the bombyx mori is raised with mulberry leaves to be mature, so that the weight of the silk gland is increased. The method achieves the increase of the weight of the silk gland of the bombyx mori by regulating the distribution of bombyx mori energy by newly discovered active polypeptides without increasing the mulberry leaf eating quantity of the bombyx mori, which has a positive effect on the study of the energy distribution mechanism of the bombyx mori, further improvement of economic characters of the bombyx mori and the like.

Description

technical field [0001] The invention relates to a synthesis of active polypeptides for increasing the weight of silk glands of silkworms, in particular to a method for increasing the weight of silk glands of silkworms. Background technique [0002] The silkworm (Bombyx mori) is the silk-producing insect with the largest amount of indoor breeding on the earth today. It absorbs various nutrients from mulberry leaves and supplies them to various tissues and organs in the silkworm through metabolic and physiological processes for growth, development, and reproduction. Nutrients are supplied to the silk glands for silk gland growth. Silk glands are the tissues and organs of silkworms that synthesize silk substances, and the quality of their growth directly affects the amount of silk produced by silkworms. For this reason, adopting various methods to promote the growth of silk glands is one of the important technical measures to improve cocoon silk production in sericulture, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/04C07K7/06C07K1/04C07K1/06
CPCA01K67/04C07K7/06
Inventor 时连根沈张飞屠振力
Owner ZHEJIANG UNIV
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