Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of composite autotrophic nitrifying bacteria, preparation method of its solid bacterial agent and its application in the sewage treatment containing high salinity and high ammonia nitrogen

A nitrifying bacteria, mixed technology, applied in biological water/sewage treatment, biochemical equipment and methods, water/sludge/sewage treatment, etc., can solve the problem of reducing sewage treatment efficiency, increasing sewage treatment cost, and low tolerance and other problems, to achieve the effect of high tolerance, fast startup and excellent performance

Active Publication Date: 2021-05-28
江苏宜裕环保科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the problems of low tolerance and low activity of traditional nitrifying bacteria in the treatment of sewage containing high salinity (salt content 1-2.1wt%) and high ammonia nitrogen (ammonia nitrogen concentration between 350-500mg / L), this problem has been reduced. The treatment efficiency of similar sewage increases the cost of sewage treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of composite autotrophic nitrifying bacteria, preparation method of its solid bacterial agent and its application in the sewage treatment containing high salinity and high ammonia nitrogen
  • A kind of composite autotrophic nitrifying bacteria, preparation method of its solid bacterial agent and its application in the sewage treatment containing high salinity and high ammonia nitrogen
  • A kind of composite autotrophic nitrifying bacteria, preparation method of its solid bacterial agent and its application in the sewage treatment containing high salinity and high ammonia nitrogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 compound autotrophic nitrifying bacteria

[0026] (1) Activation of Bacillus and Pseudomonas

[0027] The Bacillus and Pseudomonas preserved on the slant were respectively inoculated on the solid medium, and cultured in a constant temperature incubator at 30°C for 12 days to obtain activated Bacillus and Pseudomonas respectively. The Bacillus used was specific Bacillus subtilis ATCC 6633, Pseudomonas specifically Pseudomonas aeruginosa BNCC185967.

[0028] (2) Preparation of the bacterial suspension of compound autotrophic nitrifying bacteria

[0029] The expansion culture medium is divided into 250mL Erlenmeyer flasks, and the filling volume is 150mL / bottle, and the activated bacillus and pseudomonas are respectively inoculated into two rings from the solid medium to the expansion medium. In the Erlenmeyer flask, the enrichment culture was carried out on a constant temperature shaker at 30°C at 150r / min, and the culture time was 5 days...

Embodiment 2

[0035] Example 2 Composite autotrophic nitrifying bacteria and common nitrifying bacteria agent in the comparison of ammonia nitrogen degradation in artificial simulated sewage

[0036] The composite autotrophic nitrifying bacteria prepared in Example 1 and the common nitrifying bacteria agent were dropped into two identical MBBR devices respectively, and the dosage was 1‰ (m / V), and the filler in the MBBR device was a modified polyurethane filler , the dosage is 15% (V / V), artificial simulated sewage is prepared according to Table 1, the 1-4 batches are the system start-up stage, the residence time is 72h, the 5-7 batches are the ammonia nitrogen degradation stage after startup, each batch is Water 700mL, residence time 48h; control the dissolved oxygen in the two MBBR devices of each batch to 6mg / L, the temperature is 30 ℃; 4 + -N, NO 2 - -N, NO 3 - -N, the results are shown in Table 2

[0037] Table 1 Composition of artificial simulated sewage

[0038] batc...

Embodiment 3

[0043] Example 3 Comparison of the degradation of ammonia nitrogen in artificial simulated sewage by compound autotrophic nitrifying bacteria with different ratios

[0044] Group A: Mix the bacterial suspensions of Bacillus and Pseudomonas at a ratio of 1:2.5 (V / V) to obtain a high-salt compound autotrophic nitrifying bacteria suspension.

[0045] Group B: Mix the bacterial suspensions of Bacillus and Pseudomonas at a ratio of 1:1 (V / V) to obtain a high-salt compound autotrophic nitrifying bacteria suspension.

[0046] The preparation method of the solid microbial agent of group A and group B is basically the same as the preparation method of embodiment 1, the difference is only that the mixing ratio of bacillus and pseudomonas is different; the 2 batches of system startup are not listed in table 3 It is shown that the initial NH4--N in the start-up phase is 300mg / L.

[0047] Table 3 Degradation of ammonia nitrogen by compound autotrophic nitrifying bacteria with different pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a kind of compound autotrophic nitrifying bacteria, its preparation method of solid microbial agent and the application in the sewage treatment containing high salinity and high ammonia nitrogen, this compound autotrophic nitrifying bacteria is produced by Bacillus and Pseudomonas The bacteria suspension is prepared by mixing 1‑2:2.5‑5 (V / V); the number of viable bacteria contained in the Bacillus suspension is 1×10 8 -1×10 10 CFU / mL, the number of live bacteria contained in the suspension of Pseudomonas bacteria is 1×10 8 -1×10 10 CFU / mL. The compound autotrophic nitrifying bacteria of the present invention is through the interaction of Bacillus and Pseudomonas, exerts its effect in the sewage with a salt content of 1-2.1wt%, and an ammonia nitrogen concentration of 300-500mg / L, and has high tolerance. Excellent performance in ammonia oxidation and nitrite oxidation, with a removal effect of over 95%. At the same time, the microscopic examination found that the bacterial activity was always maintained at 10 8 More than CFU / mL, it has higher activity.

Description

technical field [0001] The invention belongs to the field of water treatment and environmental microorganisms, and in particular relates to a compound autotrophic nitrifying bacterium, a preparation method of its solid bacterial agent and an application in sewage treatment containing high salinity and high ammonia nitrogen. Background technique [0002] Ammonia nitrogen is the main pollutant in the water environment, and the pollution problem to the environment is very serious. In recent years, nitrifying bacteria have been widely used in the degradation of ammonia nitrogen in many fields. In the denitrification process of sewage, first, ammonia is oxidized to nitrite under the action of nitrosating bacteria, then, nitrite is oxidized to nitrate under the action of nitrifying bacteria, and finally, ammonia is oxidized to nitrate under the action of denitrifying bacteria. Nitrate is converted into gaseous nitrogen and returned to the air to purify water. Nitrification is th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/16C12R1/38C12R1/07
CPCC02F3/34C02F3/348C02F2101/16C12N1/20
Inventor 李志涛韦丽敏羊建东
Owner 江苏宜裕环保科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com