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Seed setting culture method for tissue culture seedlings of chenopodium aestivum under aseptic condition

A breeding method, quinoa group technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow hybridization process of quinoa, difficulty in artificial detasseling, etc.

Active Publication Date: 2019-09-06
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Quinoa is a floret plant (smaller and more flowers), and manual detasseling is difficult, so the hybridization process of quinoa is slow

Method used

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Examples

Experimental program
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Embodiment

[0030] A method for cultivating seeds of quinoa tissue cultured seedlings under aseptic conditions, comprising the steps of:

[0031] S1, quinoa seed treatment:

[0032] Soak quinoa seeds in 75% alcohol for 30 seconds on a clean bench, then soak them in 0.1% mercuric chloride solution for 12 minutes, and then wash them with sterile water for 3-5 times;

[0033] S2, obtaining sterile seedlings

[0034] Inoculate the above-mentioned processed quinoa seeds on the medium of 1 / 2MS+0.5mg / L CCC to prepare sterile seedlings, germinate under no light conditions until the cotyledons grow out,

[0035] in:

[0036] The culture conditions are: culture under light, temperature 25±2°C, relative humidity 70-80%, light intensity 2500Lx, light time 14h / d;

[0037] Obtain quinoa aseptic seedlings after 12 days;

[0038] S3, cutting quinoa seedlings to induce proliferation of adventitious buds

[0039] The above-mentioned quinoa tissue culture seedlings with a height of 5-6cm and 2-3 pairs ...

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Abstract

The invention discloses a seed setting culture method for tissue culture seedlings of chenopodium aestivum under the aseptic condition. The seed setting culture method includes the steps of inoculating chenopodium aestivum seeds on a 1 / 2MS+0.5mg / L CCC (chlormequat chloride) culture medium after disinfection treatment to produce seedlings and obtain aseptic seedlings of the chenopodium aestivum; cutting the aseptic seedlings of the chenopodium aestivum for proliferation and culture of adventitious buds; transferring proliferated adventitious buds into an MS+1mg / L CCC culture medium for seed setting induction. The purpose of annual seed setting of the chenopodium aestivum under the aseptic condition can be realized, and meanwhile, the seeds can be purified; during the culture process, the CCC always exists in the culture medium, the time from one seed to the seed setting of aseptic seeding is about 100-120 days which is consistent with the whole growth period of the chenopodium aestivum,infinitely many adventitious buds are proliferated and used to induce seed setting in the stage of adventitious bud proliferation, and the aseptic and pure seeds can be produced annually under the tissue culture condition for research.

Description

technical field [0001] The invention relates to the technical field of aseptic rapid propagation, in particular to a seeding cultivation method of quinoa tissue cultured seedlings under aseptic conditions. Background technique [0002] Quinoa, also known as quinoa, South American quinoa, etc., is an annual dicotyledonous plant of the family Amaranthaceae. Quinoa is a medicinal and edible crop, gluten-free, high in protein, amino acids, flavonoids, saponins and other elements, so it is called "golden grain" and is considered to be one of the crops that can ensure global food security in the future. [0003] Quinoa has been introduced and cultivated in more than 20 provinces and cities including Anhui, Gansu, Guizhou, Sichuan and Yunnan. However, the seed yield and biomass vary greatly in different regions, years and geographical conditions, mainly because the selection of quinoa varieties, variety matching, crop matching, weed control, harvesting and harvesting period manage...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 董玉梅林春毛自朝刘正杰张玉敏袁加红
Owner YUNNAN AGRICULTURAL UNIVERSITY
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