Rat culture method for transforming nonalcoholic steatohepatitis into hepatocellular carcinoma and application of method

Abstract

The invention relates to a rat culture method for transforming nonalcoholic steatohepatitis into hepatocellular carcinoma and an application of the method. The method and the application thereof effectively solve the problem that stable, reliable and effective model rats are provided for transforming nonalcoholic steatohepatitis into hepatocellular carcinoma, and technical support is provided foranimal experiments and new drug development of pathogenesis and development mechanisms of NASH, liver fibrosis, liver cirrhosis and HCC. The method includes adaptively feeding adult SD male rats weighing 250-300 g for 1 week, feeding a high-fat CDAA feed, adding diethylnitrosamine into drinking water for induction, observing mental state and hair condition of the rats, measuring intake and drinking water, and measuring weight of the rats every week; and calculating from the first feeding of high-fat CDAA diet and the addition of DEN into drinking water, and continuously culturing and feeding for 4-16 weeks to obtain rats transformed from nonalcoholic steatohepatitis to hepatocellular carcinoma. The method is easy to operate, short in time, stable and effective, and provides modeling and technical support for the research and development of new drugs for treating the transformation from nonalcoholic steatohepatitis to hepatocellular carcinoma.

Description

technical field [0001] The invention relates to the cultivation of medical animals, in particular to a method for cultivating rats transformed from non-alcoholic steatohepatitis to hepatocellular carcinoma and its application. Background technique [0002] Non-alcoholic steatohepatitis (NASH for short) has a high incidence and is very harmful. It is an important link in the development of liver fibrosis, cirrhosis, and even hepatocellular carcinoma (HCC). NASH-HCC is currently one of the leading causes of fatal liver disease. However, there are still no standardized prevention methods and treatment measures for NASH-HCC in clinical practice. Modeling methods play an important role in exploring the pathogenesis of diseases, evaluating and diagnosing, and screening drugs for prevention and treatment. A stable, high-efficiency, short-cycle, low-mortality best animal modeling method that simulates the human disease NASH-HCC is an important technical means to study its pathogen...

AI Technical Summary

Problems solved by technology

This kind of modeling can form fatty liver, liver fibrosis, NASH and HCC, but the gene knockout method is expensive and difficult to operate, which is not conducive to the smooth progress of the experiment

[0008] At present, there is no ideal animal for the transformation of NASH to HCC for modeling. Therefore, it is very necessary to provide a breeding method for the transformation of nonalcoholic steatohepatitis to hepatocellular carcinoma in rats, but there has been no public report so far.

Images