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A primer for identifying the seed purity of non-heading Chinese cabbage Sujun 316 and its application

A technology for head-heading cabbage and seeds, which is applied to the primer sequence and application field for identifying the seed purity of non-heading cabbage Sujun 316 hybrids. It can solve the problems of conflict between seed purity identification time and sales cycle, and achieve easy distinction and stability. Good results

Active Publication Date: 2022-07-05
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This variety has been using traditional field methods to identify seed purity, often causing conflicts between the timing of seed purity identification and the sales cycle

Method used

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  • A primer for identifying the seed purity of non-heading Chinese cabbage Sujun 316 and its application
  • A primer for identifying the seed purity of non-heading Chinese cabbage Sujun 316 and its application
  • A primer for identifying the seed purity of non-heading Chinese cabbage Sujun 316 and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Screening of InDel primers for purity identification

[0027] According to the genome resequencing data of Chinese cabbage, multiple pairs of primers were designed to detect the polymorphism of the three individual plants of Sujun 316, the male parent and the female parent, and the primer pair A10-2 with codominant differences was screened out. The sequence is as follows :

[0028] F: 5'-ATG CTT GGA GAT GAG ACA TGG ATC AAG-3' (SEQ ID NO: 1)

[0029] R: 5'-TTG CTC ATA TCA CCA TCA TAC AGA TAG GG-3' (SEQ ID NO: 2)

[0030] The marker has good repeatability and clear bands. The primer is used as a specific primer, and the genomic DNA of the sample to be tested is used as a template for PCR amplification, and the amplified products are separated by electrophoresis and stained for detection. A specific band of 133 bp can be amplified in medium, a specific band of 104 bp can be amplified in the parent material, and a specific band of 133 bp and 104 bp can be amplified in ...

Embodiment 2

[0042] Comparison test of traditional identification methods: The samples of Sujun 316 commercial varieties produced by 5 contracted farmers in the seed production base were randomly selected about 100 seeds from each household, and the fields were open-ditched and sown at a density of about 200 plants per square meter. About 3 days after sowing, the seedlings will emerge, and they will grow to the stage of 7 leaves and 1 heart. According to the plant height, leaf color, leaf shape, petiole color, length, width and other performance characteristics of the samples to be tested, whether they conform to the uniformity of Sujun 316 commercial varieties. To determine whether the sample to be tested is the No. 1 commodity variety of Jinyan Express, and to count the seed purity of the sample to be tested.

[0043]

[0044] Conclusion: The results of identifying the purity of Sujun 316 non-heading cabbage hybrid seeds using the present invention are consistent with the results of id...

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Abstract

The present invention discloses an InDel molecular marker specific primer for detecting the purity of Sujun 316 non-heading Chinese cabbage hybrid seeds, which is the nucleotide sequence shown in SEQ ID NO: 1-2. The invention further discloses a method for identifying the purity of Sujun 316 non-heading Chinese cabbage hybrid seeds by using specific primers. The specific primers and method provided by the present invention can complete the identification of the purity of non-heading Chinese cabbage Sujun 316 hybrid seeds in 3-5 hours in the laboratory. The identification method is beneficial to the efficient and accurate quality control of the hybrid seeds of Sujun 316 non-heading Chinese cabbage.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a primer sequence and application for identifying the purity of non-heading Chinese cabbage Sujun 316 hybrid seeds. Background technique [0002] non-heading cabbage ( Brassica rapa L. ssp. chinensis Makino) is a cruciferous Brassica crop, also known as cabbage, green cabbage, and rape (northern China). It is an important national leafy green vegetable and is also growing in growing areas abroad such as Japan and Korea. [0003] The traditional method for identifying the seed purity of non-heading cabbage is generally based on field planting. After growing to a specific period, the growth characteristics are observed to identify the purity of the seeds, which has the disadvantages of long cycle, large labor, and being easily affected by the environment. With the development of molecular biology, the application of molecular marker-assisted breeding technology in agricultural bree...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 黄志银王超楠李梅华德平张红范伟强张斌刘晓晖
Owner TIANJIN RES INST OF VEGETABLE
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