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A kind of prawn disease-resistant Toll9 protein and its coding cDNA and application

A technology for shrimp and protein, applied in the direction of anti-animal/human immunoglobulins, antiviral agents, peptide/protein components, etc., can solve the problem of sharp decline in shrimp farming production

Inactive Publication Date: 2020-08-25
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, due to a series of problems such as the deterioration of the breeding ecological environment, the frequent occurrence of non-infectious diseases and infectious diseases, the occurrence of infectious diseases (such as viral, bacterial and fungal diseases, etc.) is particularly serious, which has caused the shrimp farming industry to suffer. Considerable losses were incurred, especially when the White Spot Syndrome outbreak in 1993 resulted in a sharp drop in shrimp aquaculture production

Method used

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  • A kind of prawn disease-resistant Toll9 protein and its coding cDNA and application
  • A kind of prawn disease-resistant Toll9 protein and its coding cDNA and application
  • A kind of prawn disease-resistant Toll9 protein and its coding cDNA and application

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Embodiment Construction

[0041] The present invention will be further elaborated below through the following specific embodiments.

[0042] 1. Extraction of total RNA and construction of full-length cDNA library of hepatopancreas

[0043] 1.1 Extraction of total RNA

[0044] Fresh and live healthy Penaeus monodon (body weight about 150g) was temporarily raised in the laboratory for 3 days (water temperature was about 24°C, air pump was inflated), the shrimp body was dissected, and about 100 mg of the hepatopancreas was taken out and put into 1 mL of RLT Buffer (Qiagen, USA) Perform low-temperature grinding, extract total RNA according to the Qiagen Mini kit instructions, and use DNase digestion to remove the genome.

[0045] 1.2 Preparation of full-length cDNA template of hepatopancreas

[0046] A full-length cDNA template was prepared according to the GeneRacer kit (Invitrogen, USA). Take 3 μg of total RNA and remove the RNA 5' phosphate group by calf alkaline phosphatase (CIP) reaction, remove th...

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Abstract

The invention discloses disease-resistant Toll9 protein of prawns. The disease-resistant Toll9 protein has the amino acid sequence shown in SEQ ID NO.2. The invention further discloses cDNA for encoding the disease-resistant Toll9 protein of the prawns. The cDNA has the nucleotide sequence shown in SEQ ID NO.1. The invention further discloses an expression vector containing the cDNA and provides an antibody specifically bound with the disease-resistant Toll9 protein of the prawns. Besides, the invention further provides an application of the disease-resistant Toll9 protein of the prawns and an application of the cDNA for encoding the disease-resistant Toll9 protein of the prawns.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a disease-resistant Toll9 protein and its encoded cDNA, and the use of the disease-resistant Toll9 protein of prawns and its encoded cDNA in resisting ammonia nitrogen stress, reducing the mortality rate of prawns infected by bacteria, and in the selection and breeding of improved prawns use. Background technique [0002] my country is a big country in prawn farming. In 2005, the output of prawns cultured in my country was more than 1 million tons, accounting for about 50% of the total output of prawns in the world. In recent years, due to a series of problems such as the deterioration of the breeding ecological environment, the frequent occurrence of non-infectious diseases and infectious diseases, the occurrence of infectious diseases (such as viral, bacterial and fungal diseases, etc.) is particularly serious, which has caused the shrimp farming industry to suffer. Co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C12N15/12C07K16/18A61K38/17A61P37/04A61P31/04A61P31/12
CPCA61K38/00C07K14/43509C07K16/18
Inventor 杨丽诗江世贵黄建华周发林马振华杨其彬姜松
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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