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Application of chromatin remodeling factor genes in saccharomyces cerevisiae in increasing yield of fermentation alcohol

A technology for Saccharomyces cerevisiae and fermenting alcohol, which is applied in the field of bioenergy technology development, can solve the problem of not improving the alcohol yield of Saccharomyces cerevisiae, and achieve the effects of improving production efficiency and improving alcohol yield

Pending Publication Date: 2019-04-23
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on improving the alcohol yield of Saccharomyces cerevisiae by chromatin remodeling method

Method used

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  • Application of chromatin remodeling factor genes in saccharomyces cerevisiae in increasing yield of fermentation alcohol
  • Application of chromatin remodeling factor genes in saccharomyces cerevisiae in increasing yield of fermentation alcohol

Examples

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Effect test

Embodiment 1

[0018] Embodiment 1SPT10, the screening method of SWI3, NGG1, UME6 and RSC1

[0019] From the diploid Saccharomyces cerevisiae gene deletion strain library (Invitrogen Inc, U.S.), select 150 single-gene deletion strains of genes related to gene transcription, carry out alcoholic fermentation experiments to them according to the method of Example 2 below, and find SPT10, SWI3, NGG1, UME6 and RSC1, the deletion strains of five chromatin remodeling factor genes, had significantly higher ethanol production than wild-type strains. The gene sequences of SPT10, SWI3, NGG1, UME6 and RSC1 are shown in SEQ ID NO.1-5.

Embodiment 2

[0020] The alcoholic fermentation of embodiment 2 saccharomyces cerevisiae

[0021] The experimental strains were activated by streaking on the YPD plate and cultured at 30°C for 2 days. Take a single colony from each strain and inoculate 30 mL of YPD liquid medium, culture at 30°C and shaker at 220 r / min for more than 16 hours until saturated. Inoculate the seed bacteria liquid into 100mL of fermentation medium at a ratio of 10%, carry out shaker culture at 30°C and 220r / min for 8 hours, and then transfer to static culture for fermentation.

[0022] Among them, the seed medium (YPD) contains 2% glucose, 1% yeast extract, 2% peptone, dissolved in deionized water, and has a pH of 7.0. The content (g / L) of various components in the fermentation medium is: glucose 100, ammonium sulfate 7.5, potassium dihydrogen phosphate 3.5, magnesium sulfate heptahydrate 0.75, yeast extract 0.2, histidine 0.02, uracil 0.02, bright Amino acid 0.1, pH7.0. Both media were sterilized by autoclav...

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Abstract

The invention discloses application of chromatin remodeling factor genes in saccharomyces cerevisiae in increasing the yield of fermented alcohol. The chromatin remodeling factor genes comprise SPT10,SWI3, NGG1, UME6 and RSC1 genes, and the nucleotide sequences are shown in SEQ ID NO.1-5. A new method is provided for improving the production efficiency of alcohol fermentation, and important information is provided for constructing an excellent saccharomyces cerevisiae engineering strain of high-yield alcohol.

Description

technical field [0001] The invention belongs to the technical field of bioenergy technology development, and in particular relates to the application of a chromatin remodeling factor gene in Saccharomyces cerevisiae to increase the yield of fermented alcohol. Background technique [0002] The production of fuel alcohol by microbial fermentation is a hot spot in current research, and Saccharomyces cerevisiae is currently the most ideal alcohol fermentation production strain. At present, the technology of producing fuel alcohol through fermentation of Saccharomyces cerevisiae using cornstarch as raw material has been very mature at home and abroad. The traditional means of strain selection (such as natural selection, mutation breeding) to improve the alcohol fermentation yield of strains has basically reached the limit. In recent years, with the rapid development of molecular biology and omics technology, conditions have been provided for large-scale research on the relations...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12R1/865
CPCC12P7/06Y02E50/10
Inventor 蒋伶活
Owner SHANDONG UNIV OF TECH
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