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Modified illite powder microbial agent as well as preparation method and application thereof

A technology of microbial bacterial agent and illite powder, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of single type of heavy metals and few applications, so as to reduce biological effectiveness, simplify operation steps, and strengthen The effect of adsorption capacity

Active Publication Date: 2019-04-05
湖南慧谷农业生态研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves on existing modifying litters made from activated charcoal for better use against heavy metals like Cadmium or Cd. By adding these modifications into litter particles instead of just mixing them together, they become more effective at reducing environmental pollution caused by harmful substances found naturally occurring throughout their lifespan (living). Additionally, this modification allows other materials that may help improve functionality within the litter itself to actively participate in redox processes, promoting cellular activity without being consumed quickly enough due to reduced levels of free radicals produced therein. Overall, this innovative improvement enhance the benefits associated with improved litter products while also minimizing negative impacts upon ecologically sensitive environments where indoor agriculture needs to rely heavily on pesticides containing chelating compounds.

Problems solved by technology

This patented describes different methods that have been developed specifically for treating heavily loaded environments containing harmful substances like Cadmiums, Pb, Hg, Nitrates, Coptods, Chelatites, Fe(III)-Cotropes, Thallium polysulfonic acid (TSPS). These techniques aim at reducing these harms while maintaining beneficial crop crops. However, current technologies require expensive equipment and complicated processes due to their complexity nature and limited applicability range.

Method used

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  • Modified illite powder microbial agent as well as preparation method and application thereof
  • Modified illite powder microbial agent as well as preparation method and application thereof
  • Modified illite powder microbial agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 A kind of preparation method of modified illite powder microbial bacterial agent

[0034] Specifically include the following steps:

[0035] Step 1, the preparation of the adsorption carrier, dissolve a certain amount of carboxymethyl chitosan in water, stir until the solution is transparent, then add illite powder, and the mass ratio of the two satisfies 8:1, stir for 30 minutes after fully infiltrating, add mass The ethanol solution with a fraction of 80% was used to obtain a mixed condensate, which was washed three times with deionization, filtered and dried to obtain an adsorption carrier;

[0036] Step 2, preparation of the mixed bacteria fermentation product, the specific steps include:

[0037] a. Slant culture: inoculate Achromobacter xylosoxidans, Sphingomonas paucimobilis, and Bacillus megaterium on the slant medium under aseptic conditions respectively, and culture at 30°C for 24 hours;

[0038] b. Cultivation on a shaker: Inoculate the slant s...

Embodiment 2

[0043] Embodiment 2 A kind of preparation method of modified illite powder microbial bacterial agent

[0044] Specifically include the following steps:

[0045] Step 1, the preparation of the adsorption carrier, dissolve a certain amount of hydroxypropyl chitosan in water, stir until the solution is transparent, then add illite powder, and the mass ratio of the two satisfies 5:1, stir for 20 minutes after fully infiltrating, add mass Ethanol solution with a fraction of 70% to obtain a mixed condensate, washed three times with deionization, filtered and dried to obtain an adsorption carrier;

[0046] Step 2, preparation of the mixed bacteria fermentation product, the specific steps include:

[0047] a. Slant culture: inoculate Achromobacter xylosoxidans, Sphingomonas paucimobilis, and Bacillus megaterium on the slant medium under aseptic conditions respectively, and culture at 30°C for 24 hours;

[0048] b. Cultivation on a shaker: Inoculate the slant strains cultivated in st...

Embodiment 3

[0053] Embodiment 3 A kind of preparation method of modified illite powder microbial bacterial agent

[0054] Specifically include the following steps:

[0055] Step 1, the preparation of the adsorption carrier, dissolve a certain amount of small molecular chitosan in water, stir until the solution is transparent, then add illite powder, and the mass ratio of the two satisfies 8:1, stir for 30 minutes after fully infiltrating, add the mass fraction 80% ethanol solution to obtain a mixed condensate, washed three times with deionization, filtered and dried to obtain an adsorption carrier;

[0056] Step 2, preparation of the mixed bacteria fermentation product, the specific steps include:

[0057] a. Slant culture: inoculate Achromobacter xylosoxidans, Sphingomonas paucimobilis, and Bacillus megaterium on the slant medium under aseptic conditions respectively, and culture at 30°C for 24 hours;

[0058] b. Cultivation on a shaker: Inoculate the slant strains cultivated in step a...

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Abstract

The invention discloses a modified illite powder microbial agent as well as a preparation method and application thereof. The modified illite powder microbial agent is prepared from the following components in parts by weight: 75-85 parts of an adsorption carrier and 15-25 parts of a mixed strain fermentation product, wherein the adsorption carrier is prepared by loading illite powder with water-soluble chitosan or a derivative of the water-soluble chitosan and then modifying the illite powder through ethanol, the mixed strain fermentation product is prepared by carrying out slant culture andshake culture on original strains of achromobacter xylosoxidans, sphingomonas paucimobilis and bacillus megatherium respectively and sequentially under the sterile condition, then inoculating a liquidfermentation medium with the original strains jointly, carrying out mixed culture through a fermentation tank, and then carrying out centrifuging, concentrating and drying, and the viable count of the mixed strain fermentation product is 20-30 billion cfu/ml. According to the modified illite powder microbial agent as well as the preparation method and application thereof, a product is applied during soil preparation, heavy metal ions can be immobilized in situ, the biological effectiveness of the heavy metal cadmium in soil can be effectively reduced, and the absorption of crops to the heavymetal cadmium ions can be reduced.

Description

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Claims

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Application Information

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Owner 湖南慧谷农业生态研究院有限公司
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