Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of multifunctional hyaluronic acid lipidosome and quality control standard

A hyaluronic and multifunctional technology, applied in the field of drug research, can solve the problems of hyaluronic acid liposomes with large molecular weight and hyaluronic acid not being able to penetrate the skin well

Inactive Publication Date: 2019-03-01
TIANJIN POLYTECHNIC UNIV
View PDF9 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in terms of practical application, due to the relative molecular weight of the hyaluronic acid liposome itself, the exogenously supplemented hyaluronic acid cannot penetrate the skin well, so it cannot fundamentally play a role

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Accurately weigh 0.3g of lecithin (PC) and 0.003g of cholesterol (CH) into a round bottom flask, dissolve it in 30ml of petroleum ether / methanol mixed solvent with a volume ratio of 2:1; weigh 0.009g of hyaluronic acid The acid (HA) was dissolved in 10ml of PBS solution with a pH of 6.6, and the previously prepared solutions were combined and ultrasonically treated for 10 minutes to obtain a W / O emulsion; then the organic solvent was removed by rotary evaporation under reduced pressure to obtain a semi-solid gel-like substance Continue to add 10ml of PBS buffer solution, help the gel on the wall of the device to come off by continuous rotary evaporation, sonicate for 10min, and finally obtain a liposome suspension.

[0018] Results: The encapsulation efficiency of the prepared hyaluronic acid liposome was 19.5%.

Embodiment 2

[0020] Accurately weigh 0.3g of lecithin (PC) and 0.003g of cholesterol (CH) into a round bottom flask, dissolve it in 30ml of petroleum ether / methanol mixed solvent with a volume ratio of 2:1; weigh 0.009g of hyaluronic acid The acid (HA) was dissolved in 10ml of PBS solution with a pH of 6.8, and the previously prepared solutions were combined, and ultrasonically treated for 10 minutes to obtain a W / O emulsion; then the organic solvent was removed by rotary evaporation under reduced pressure to obtain a semi-solid gel-like substance Continue to add 10ml of PBS buffer solution, help the gel on the wall of the device to come off by continuous rotary evaporation, sonicate for 10min, and finally obtain a liposome suspension.

[0021] Results: The encapsulation efficiency of the prepared hyaluronic acid liposome was 18.3%.

Embodiment 3

[0023] Accurately weigh 0.3g of lecithin (PC) and 0.003g of cholesterol (CH) into a round bottom flask, dissolve it in 30ml of petroleum ether / methanol mixed solvent with a volume ratio of 2:1; weigh 0.009g of hyaluronic acid The acid (HA) was dissolved in 10ml of PBS solution with a pH of 7.0, and the previously prepared solutions were combined and ultrasonically treated for 10 minutes to obtain a W / O emulsion; then the organic solvent was removed by rotary evaporation under reduced pressure to obtain a semi-solid gel-like substance Continue to add 10ml of PBS buffer solution, help the gel on the wall of the device to come off by continuous rotary evaporation, sonicate for 10min, and finally obtain a liposome suspension.

[0024] Results: The encapsulation efficiency of the prepared hyaluronic acid liposome was 18.6%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method of a multifunctional hyaluronic acid lipidosome and a quality control standard. The method comprises the following steps: (1) weighing phosphatidylcholine (PC), cholesterol (CH) and hyaluronic acid (HA) according to a proportion of 0.1 to 0.03 to 1, dissolving the PC and CH in a mixed solution prepared from petroleum ether / methanol in a volume ratio of 2to 1, dissolving the HA in phosphate buffered saline (PBS), and performing ultrasonic treatment for later use; (2) combining the products obtained in the step (1) to form emulsion, and performing reduced-pressure rotary evaporation for removing an organic solvent to obtain a semi-solid gel substance; (3) adding the PBS into the semi-solid gel substance obtained in the step (2), continually performing rotary evaporation to assist the falling of gel on the wall of a device, and performing ultrasonic treatment to obtain a lipidosome suspension; (4) calculating the encapsulation efficiency of thelipidosome to serve as a target value for evaluating the index of the lipidosome quality. By adopting the method, the hyaluronic acid is conveyed to the keratoderma better, and can act stably.

Description

technical field [0001] The invention provides a preparation method and a quality inspection standard of a multifunctional hyaluronic acid liposome, belonging to the field of drug research. technical background [0002] The skin is the first external protective layer of the human body. Moisturizing is the primary task in skin care, because the moisture of the skin is very easy to lose, and the lack of sufficient moisture in the skin will directly lead to abnormal skin metabolism, aging, wrinkles and spots And so on a series of skin problems. However, factors such as air-conditioning, environmental pollution, temperature changes and pressure caused by seasonal changes, skin aging, and metabolic slowdown will all cause skin moisture loss. Generally speaking, moisturizing the skin is the water holding capacity of the skin, which can slow down the speed of evaporation and loss of water in the skin. [0003] Hyaluronic acid has a special water-retaining effect. It is the most mo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K8/73A61K8/14A61Q19/00
CPCA61K8/14A61K8/735A61Q19/00
Inventor 郭文娟朱大伟于洁殷明慧
Owner TIANJIN POLYTECHNIC UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com