Method for preparing bio-organic fertilizer by utilizing corn steep liquor and lysine fermentation waste liquid
A technology of lysine fermentation and bio-organic fertilizer, which is applied in microorganism-based methods, preparation of organic fertilizers, treatment of bio-organic parts, etc. The effect of income
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Embodiment 1
[0023] 1) Put the lysine fermentation waste liquid in the separation equipment, mix, cool down and generate supersaturation, and precipitate crystals to obtain liquid fertilizer mother liquor; Bacillus subtilis and Bacillus licheniformis are mixed according to the weight ratio of 1:1 to obtain mixed biological bacteria agent;
[0024] 2) Using corn steep liquor and liquid fertilizer mother liquor to prepare a liquid medium, the medium includes the following raw materials according to weight percentage: corn steep liquor 17%, liquid fertilizer mother liquor 12%, calcium chloride 0.58%, glucose 6%, and the balance is water;
[0025] 3) Transfer the culture medium into a fermenter, add 0.001% medium quality mixed biological agent, and cultivate it for 18 hours at 37°C, with a pressure of 0.1Mpa and an air volume of 0.2vvm, to obtain a spore content of 10 billion / g bacterial liquid;
[0026] 4) Mix corn steep liquor, liquid fertilizer mother liquor, and bacterial liquor according...
Embodiment 2
[0028] 1) Put the lysine fermentation waste liquid in the separation equipment, mix, cool down and generate supersaturation, and precipitate crystals to obtain liquid fertilizer mother liquor; Bacillus subtilis and Bacillus licheniformis are mixed according to the weight ratio of 3:4 to obtain mixed biological bacteria agent;
[0029] 2) Using corn steep liquor and liquid fertilizer mother liquor to prepare liquid culture medium, the culture medium includes the following raw materials according to weight percentage: corn steep liquor 18%, liquid fertilizer mother liquor 9%, calcium chloride 0.48%, glucose 4.7%, and the balance is water;
[0030] 3) Transfer the culture medium into a fermenter, add 0.001% of the medium quality mixed biological agent, and cultivate it for 18 hours at 41°C, with a pressure of 0.1Mpa and an air volume of 0.2vvm, to obtain a spore content of 11.5 billion / g bacterial liquid;
[0031] 4) Mix corn steep liquor, liquid fertilizer mother liquor, and ba...
Embodiment 3
[0033] 1) Put the lysine fermentation waste liquid in the separation equipment, mix, cool down and generate supersaturation, and precipitate crystals to obtain liquid fertilizer mother liquor; mix Bacillus subtilis and Bacillus licheniformis according to the weight ratio of 3:2 to obtain mixed biological bacteria agent;
[0034] 2) Using corn steep liquor and liquid fertilizer mother liquor to prepare a liquid medium, the medium includes the following raw materials according to weight percentage: 20% corn steep liquor, 10% liquid fertilizer mother liquor, 0.5% calcium chloride, 5% glucose, and the balance is water;
[0035] 3) Transfer the culture medium into a fermenter, add 0.001% medium quality mixed biological agent, and cultivate it for 18 hours at 40°C, with a pressure of 0.1Mpa and an air volume of 0.2vvm, to obtain a spore content of 12 billion / g bacterial liquid;
[0036] 4) Mix corn steep liquor, liquid fertilizer mother liquor, and bacterial liquor according to the...
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