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Application of intestinal microorganisms as diagnostic markers of cholangiocarcinoma

A technology of intestinal microbes and cholangiocarcinoma, which is applied in the field of microbial markers of cholangiocarcinoma, and can solve the problems that there are no studies on intestinal flora biomarkers of cholangiocarcinoma

Active Publication Date: 2019-01-25
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently, no studies have elucidated gut microbiota biomarkers associated with cholangiocarcinoma

Method used

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  • Application of intestinal microorganisms as diagnostic markers of cholangiocarcinoma
  • Application of intestinal microorganisms as diagnostic markers of cholangiocarcinoma
  • Application of intestinal microorganisms as diagnostic markers of cholangiocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Detection of differences in the abundance of intestinal microbial flora in cholangiocarcinoma, hepatocellular carcinoma, liver cirrhosis, and healthy people

[0060] 1. Research object

[0061] Patients with cholangiocarcinoma, hepatocellular carcinoma and liver cirrhosis were collected and confirmed by liver biopsy.

[0062] The patient information and healthy person information are shown in Table 1.

[0063] Table 1 Clinical information

[0064]

[0065]

[0066] 2. Feces collection and treatment

[0067] Fresh, middle and late stool samples were collected and immediately frozen in a -80°C freezer.

[0068] 3. Nucleic acid extraction

[0069] Stool sample DNA according to MOBIO DNA Isolation Kit 12888-100 instruction manual for extraction. The general steps are as follows:

[0070] (1) Add 0.25g of feces sample to a powerBead Tubes, vortex gently;

[0071] (2) Add 60μl Solution C1, mix up and down several times;

[0072] (3) Fix the powerBead...

Embodiment 2

[0110] Example 2 Clinical diagnostic value of Lactobacillus and Alloscardovia

[0111] According to the abundance data obtained in Example 1, a ROC curve was made to analyze the clinical diagnostic value of Lactobacillus and Alloscardovia.

[0112] result:

[0113] image 3 The results showed that when Lactobacillus genus differentiated cholangiocarcinoma and hepatocellular carcinoma, the AUC was 0.429;

[0114] Figure 4 When showing Lactobacillus to distinguish cholangiocarcinoma from liver cirrhosis, the AUC was 0.729;

[0115] Figure 5 When showing Lactobacillus to distinguish cholangiocarcinoma from normal people, the AUC was 0.679;

[0116] Figure 6 When showing Alloscardovia genus to distinguish cholangiocarcinoma from hepatocellular carcinoma, the AUC was 0.798;

[0117] Figure 7 When showing Alloscardovia genus to distinguish cholangiocarcinoma from liver cirrhosis, the AUC was 0.729;

[0118] Figure 8 When showing Alloscardovia to distinguish cholangioc...

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Abstract

The invention discloses the application of intestinal bacteria in feces in the screening, diagnosis or auxiliary diagnosis of cholangiocarcinoma. The invention obtains the characteristics of bacterialspecies and abundance by extracting DNA of bacteria in feces and sequencing, and carries out diagnosis of cholangiocarcinoma on the basis of the bacterial abundance characteristics. Compared with thecurrent method for screening cholangiocarcinoma which has been used in clinic or patent application, the present invention is completely non-invasive and can more accurately predict and diagnose cholangiocarcinoma.

Description

technical field [0001] The invention relates to the fields of biotechnology, disease diagnosis and biomedicine, in particular to microbial markers of cholangiocarcinoma and applications thereof. Background technique [0002] Liver space-occupying lesions can be benign or malignant tumors, and malignant tumors can be metastatic or primary. Primary liver cancer (primary carcinoma of the liver) is one of the common malignant tumors in my country. The main types of primary liver cancer are hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC). At present, the principle of pathological diagnosis for patients with primary liver cancer after surgery is mainly based on histological examination of surgically resected tumor tissue and clinical examination of patients. Hepatocellular carcinoma and intrahepatic cholangiocarcinoma are often difficult to diagnose due to their similar histological morphology and cross-immune phenotypes; on the other hand, the treatment...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/04G01N33/569C12R1/01C12R1/225
CPCC12Q1/689G01N33/56916
Inventor 贾晓东陆荫英卢姗姗
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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