Cells loaded with gold nanoparticles for use in the diagnosis and/or treatment of melanoma

A nanoparticle and cell technology, which is used in preparations for in vivo experiments, wave energy or particle radiation treatment materials, antitumor drugs, etc.

Active Publication Date: 2019-01-15
UNIVERSITY OF FLORENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition to off-target toxicity, another problem that has not been overcome is the clearance of metal nanoparticles once their diagnostic and / or therapeutic effects are complete
[0009] For all of the above reasons, metal nanoparticles have not yet migrated from use in animal models to practical use in humans as diagnostic and / or therapeutic agents, despite great expectations from the scientific community in recent years.

Method used

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  • Cells loaded with gold nanoparticles for use in the diagnosis and/or treatment of melanoma
  • Cells loaded with gold nanoparticles for use in the diagnosis and/or treatment of melanoma
  • Cells loaded with gold nanoparticles for use in the diagnosis and/or treatment of melanoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 - Synthesis of Gold Nanoparticles

[0043] At room temperature (25°C), 0.5ml Na 2 S 2 o 3 The aqueous solution was quickly added to 2.5ml HAuCl 4 In aqueous solution, the gold / thiosulfate molar ratio is 2.6. The solution was kept on a vortex mixer for 20 seconds and then allowed to stand for about 10 minutes, during which time a color change was observed, from yellow to brown to dark red. Completion of the reaction was confirmed by recording the UV-Vis spectrum of the solution after 30 minutes and then again at a time interval of 10 minutes. 0.09 ml of 0.1% (w / v) high molecular weight chitosan (~10 6 Da; degree of deacetylation 79%) in acetic acid solution (concentration 1% (v / v)). The final concentration of gold was 1.4 mM. The colloidal solution thus obtained is then left under slow stirring for 6-12 hours and then autoclaved.

[0044] The preparation process described above was repeated by varying the gold / thiosulfate molar ratio, in particular wi...

Embodiment 2

[0046] Example 2 - Characterization of Gold Nanoparticles and Chitosan Solutions

[0047] The colloidal solution obtained as described in Example 1 above has been studied to evaluate the stability over time under continuous irradiation, thermal conductivity and photothermal stability.

[0048] Still based on the UV-Vis absorption spectrum, the stability over time of the solution prepared as described above can be confirmed by recording the same solution freshly prepared and after a period of 3 weeks of maturation, whereby essentially superimposed spectra are obtained, as figure 2 shown. Similar results were obtained by extending the maturation time of the colloidal solution to 15 weeks after preparation.

[0049] In addition, by using a laser with a wavelength of 808 nm and two different laser irradiation intensity levels (i.e., 1W / cm 2 and 2W / cm 2 ) irradiated the solution, and by measuring the rise of the temperature of the irradiated solution, the thermal conductivity...

Embodiment 3

[0051] The separation of embodiment 3-ECFC cells and mesenchymal stem cells (MSC)

[0052] According to Italian law, after the mother's free, informed and written consent, the umbilical cord blood unit (hereinafter referred to as UBC), in which the total number of nucleated cells 9 (Appropriate threshold for umbilical cord inclusions from a hospital bank established at the Careggi Umbilical Cords Bank in Florence, Italy). ECFC cells were isolated from UBC according to the method described by Margheri F. et al. (Blood. 2011; 118:3743-55). Cells were characterized as expressing the selectable markers CD34, CD133 and not expressing CD45, and the appearance of specific endothelial markers (KDR, CD144, CD141, CD105, VWF, CD31) was monitored by flow cytometry.

[0053] The MSCs used as comparisons in the following examples were obtained from aspirates of hematopoietic bone marrow (hereinafter referred to as BM) of patients who provided their free and informed written consent. Tot...

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Abstract

The present invention refers to cells from a subpopulation of progenitors of endothelial cells loaded with gold nanoparticles sensitive to excitation with infrared radiation with consequent release ofthermal energy, to a composition comprising a plurality of these cells and to their use in the diagnosis and treatment of solid tumours thanks to the selective localization of the composition in thetumour mass, which can be thus identified easily for diagnostic purposes by marking the cells with appropriate marking agents, or treated by stimulation with pulsed laser beam up to the complete cauterization of the tumour mass for a targeted and highly effective therapeutic action.

Description

technical field [0001] The present invention relates generally to the field of pharmaceuticals, and more specifically to subpopulations of endothelial progenitor cells labeled with a labeling agent and loaded with gold nanoparticles, and their usefulness in the diagnosis and treatment of melanoma and its possible metastases and other Use in Solid Tumors. Background technique [0002] Since the phenotype of cancer cells is often similar to that of normal cells (often the existing differences are more quantitative than qualitative) and varies greatly between different tumor types, the most difficult problem to overcome in oncology One of the important challenges is to identify specific tumor molecular targets. [0003] Recent scientific advances in the field of cellular and molecular biotechnology have opened up new and advanced therapeutic options, such as somatic gene therapy and nanomedicine, for the treatment of various diseases for which the treatment is not always effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K41/00A61K49/00A61K51/12A61K33/242A61P35/00A61P35/04
CPCA61K41/0052A61K49/0065A61K47/6901A61K51/1203A61P35/00A61K35/28A61K33/242
Inventor 马里奥·德尔·罗索安娜·劳伦扎纳加布里埃尔·菲比弗朗西斯卡·马尔盖里阿纳斯塔西娅·奇拉安琪拉·佐皮吉安卡洛·马尔盖里
Owner UNIVERSITY OF FLORENCE
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