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Culture method of soft seed pomegranate young seedlings

A cultivation method and seedling technology are applied in the field of cultivation of soft-seeded pomegranate seedlings, which can solve the problems of low survival rate, long growth cycle of pomegranate seedling cultivation and low pomegranate yield, and achieve high survival rate, accelerated initial growth and survival rate, The effect of improving quality and yield

Inactive Publication Date: 2019-01-15
会泽高老庄农业庄园有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the traditional method, the cultivation and growth cycle of pomegranate seedlings is longer, and the survival rate is low, so that the yield of pomegranate is relatively low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for cultivating soft-seed pomegranate seedlings, comprising the steps of:

[0023] (1) Selection and pretreatment of seedlings: use the young shoots extracted from pomegranate japonica in that year as explants, and treat them with 62% alcohol for 22 seconds, 8.3% NaClO solution for 23 minutes, place them for 19 hours, and then use 0.06 %HgCl 2 Disinfect for 16 minutes;

[0024] (2) Initial development of seedlings: After disinfection, the explants with axillary buds were cut into 7cm segments, placed on MS medium formula compounded with 6.2g / L agar, 25g / L and phytic acid 1.5mg / mL, grow on culture medium;

[0025] (3) Rapid propagation of seedlings: Spray natural additives within 3 days of initial development, then spray natural additives and exogenous additives within 5 days, and then spray 6-BA in the next 2 days to grow into tissue seedlings;

[0026] (4) Transplanting of the tissue seedlings: the tissue seedlings are moved to the nursery for cultivation, ...

Embodiment 2

[0035] A method for cultivating soft-seed pomegranate seedlings, comprising the steps of:

[0036] (1) Selection and pretreatment of seedlings: use the young shoots of pomegranate japonica that were extracted in that year as explants, and treat them with 64% alcohol for 25 seconds, 9% NaClO solution for 25 minutes, place them for 21 hours, and then use 0.07 %HgCl 2 Disinfect for 17 minutes;

[0037] (2) Initial development of seedlings: After disinfection, the explants with axillary buds were cut into 7cm segments, and placed in MS medium formulated with 6.5g / L agar, 26g / L and phytic acid 2mg / mL. grow on culture medium;

[0038] (3) Rapid propagation of seedlings: Spray natural additives within 3 days of initial development, then spray natural additives and exogenous additives within 5 days, and then spray 6-BA in the next 2 days to grow into tissue seedlings;

[0039] (4) Transplanting of the tissue seedlings: the tissue seedlings are moved to the nursery for cultivation, ...

Embodiment 3

[0048] A method for cultivating soft-seed pomegranate seedlings, comprising the steps of:

[0049] (1) Selection and pretreatment of seedlings: use the young shoots extracted from the pomegranate in the same year as explants, and treat them with 68% alcohol for 28 seconds, 9.5% NaClO solution for 27 minutes, place them for 22 hours, and then use 0.05 ~0.1%HgCl 2 Disinfect for 17 minutes;

[0050] (2) Initial development of seedlings: After disinfection, the explants with axillary buds were cut into 8.5cm segments, placed in MS medium formula compounded with 6.7g / L agar, 26.5g / L and 2.7mg / L phytic acid. mL, grow on culture medium;

[0051] (3) Rapid propagation of seedlings: Spray natural additives within 3 days of initial development, then spray natural additives and exogenous additives within 5 days, and then spray 6-BA in the next 2 days to grow into tissue seedlings;

[0052](4) Transplanting of the tissue seedlings: the tissue seedlings are moved to the nursery for cult...

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Abstract

The invention relates to the technical field of agricultural planting, in particular to a culture method of soft seed pomegranate young seedlings. The method comprises the following steps of selectionand pretreatment of young seedlings, initial development of young seedlings, fast breeding of young seedlings and transplanting of tissue seedlings. The method has the advantages that the breeding speed is high; the bud survival rate is high; plant diseases and insect pests can be effectively inhibited; the healthy and strong growth condition can be provided for young seedlings; after the transplanting, the survival rate is high; the transplanting survival rate reaches 98 percent; the young seedlings are sterilized; bacteria inside explants can be effectively killed; meanwhile, the seed surface mycete growth can be inhibited; the occurrence of plant diseases and insect pests can be reduced, so that the pomegranate young seedling survival rate is high; through natural additives, external additives and 6-BA, the early stage growth condition of the seedlings is obviously accelerated; the survival rate is improved; the quality and the yield of the pomegranate, the grade and leaf quality are improved; mixed liquid of coconut juice and sorghum young spike extraction liquid and the like are used, so that the seedlings fast grow and develop; in addition, the browning phenomenon due to browning of the seedling explants is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a method for cultivating soft-seed pomegranate seedlings. Background technique [0002] Pomegranate is a deciduous shrub or small tree. After its fruit matures, it becomes a large, multi-locular, multi-seed berry, with many seeds in each cell; the outer testa is fleshy, bright red, light red or white, juicy, sweet and tinged Sour, that is, the edible part, the inner testa is cutin, and there are also degenerated and soft ones, that is, soft seed pomegranate. Pomegranate is sweet, sour and warm, and has the effects of killing insects, astringent, astringent intestines, and stopping dysentery. Pomegranate fruit is rich in nutrients, and the vitamin C content is one or two times higher than that of apples and pears. Pomegranate is particularly rich in nutrients and contains a variety of nutrients needed by the human body. The fruit contains vitamin C and B vitamins, o...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高明绍高明静高豪高伟杰
Owner 会泽高老庄农业庄园有限公司
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