Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of integrin beta4 gene DNA methylation site to preparation of biomarkers for early diagnosis of asthma and/or COPD

A biomarker and early diagnosis technology, applied in the field of biology, can solve problems such as inability to cure and no risk prediction markers yet

Active Publication Date: 2019-01-08
CENT SOUTH UNIV
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, the morbidity and mortality of asthma and COPD have been increasing year by year in recent years, and they still cannot be cured clinically, causing serious family economic burden and social health problems
However, asthma and COPD are complex diseases caused by the interaction of polygenic inheritance and environmental factors, so far there is no effective clinical risk prediction marker

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of integrin beta4 gene DNA methylation site to preparation of biomarkers for early diagnosis of asthma and/or COPD
  • Application of integrin beta4 gene DNA methylation site to preparation of biomarkers for early diagnosis of asthma and/or COPD

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] (1) Use MethylTargetTM methylation site evaluation technology to evaluate, clarify the CpG island and other sequence information of integrin β4, provide information for determining candidate research regions, and estimate the number of fragments and systems required.

[0014] Evaluation Criteria

[0015] A. Find the CpG island in the region from 2K upstream of the gene transcription start site to 1K downstream of the first exon (including all splicing modes).

[0016] The parameters for the assessment of methylated CpG islands are:

[0017] Observed / Expected ratio>0.60, Percent C+Percent G>50.00%, Length>200bp.

[0018] B. Fragment Number Evaluation

[0019] a) Conventional (default assessment): each CpG island is expected to design a pair of primers for detection, for longer CpG islands increase (200bp-1K, 1 pair, 1K-2K, 2 pairs, and so on);

[0020] b) Full coverage: calculated according to the length of each CpG island;

[0021] c) Customer-designated interval: d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of an integrin beta4 gene DNA methylation site to the preparation of biomarkers for early diagnosis of asthma and / or COPD.

Description

technical field [0001] The invention belongs to the technical field of biology, and relates to the application of integrin β4 gene DNA methylation site in the preparation of biomarkers for the early diagnosis of asthma and / or COPD, in particular to a biological method for the early diagnosis of asthma and / or COPD Markers and detection methods. Background technique [0002] Bronchial asthma (asthma) and COPD (chronic obstructive pulmonary disease) are common diseases of the respiratory system, but so far the clinical treatment effect of asthma and COPD is unsatisfactory and still cannot be cured, causing great economic and psychological burden to patients , has become a public health issue of global concern. A survey found that the mortality and morbidity of adult asthma and COPD have been increasing in the past 30 years, accounting for 1% of the total global disease burden. It is estimated that asthma, COPD-related lung disease will become the third leading cause of human ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/154
Inventor 刘持秦岭秦晓群袁琳唐莎杜茜子
Owner CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com