Adjustable-temperature solid phase synthesis apparatus for polypeptide

Abstract

The invention discloses adjustable-temperature solid phase synthesis apparatus for polypeptide. The apparatus comprises a kettle body; the upper end of the kettle body is provided with a material inlet, the lower end of the kettle body is provided with a material outlet, and the interior of the kettle body is provided with a stirring a unit; the stirring unit comprises a plurality of stirring devices, each stirring device comprises one rotating sleeve shaft, each rotating sleeve shaft comprises one rotating shaft and one driving shaft arranged in the rotating shaft in a sleeving manner, two ends of each driving shaft are separately connected with one driving unit located at the exterior of the kettle body, and a plurality of stirring blades are uniformly arranged on the periphery of each rotating shaft; and the lower end of the kettle body is externally connected with a vacuuming unit, and a temperature control unit is arranged in the kettle body. The apparatus disclosed by the invention has a simple and clear structure, the components are closely connected with one another and do not affect one another, and the required components are convenient to replace, so that repairing and maintenance of the product in the using process in the future are facilitated.

Description

technical field [0001] The invention relates to the field of chemical equipment, in particular to a temperature-adjustable polypeptide solid-phase synthesis device. Background technique [0002] Polypeptide solid-phase synthesis is to connect the protected amino acid to a solid resin, remove the protective group, then react the next protected amino acid with the residue of the first amino acid and form a peptide bond, and then remove the protective group of the second amino acid group, and then connect to the next amino acid, so that the cycle continues to increase the length of the peptide chain. In the solid-phase synthesis of polypeptides, solid resin is the necessary reaction substrate. In the peptide solid-phase synthesis reaction, temperature has a great influence on the synthesis result. Most of the peptide synthesizers used in the prior art synthesize according to the temperature outside the instrument, and the synthesis effect is not ideal, or there are instruments...

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Problems solved by technology

[0003] In order to solve the above problems, the present invention discloses a temperature-adjustable polypeptide solid-phase synthesis device, the purpose of which is to solve the problems of high cost of temperature control and complex structure in the process of polypeptide synthesis

The design structure is simple and clear, the components are closely connected without interfering with each other, the required parts are easy to replace, and it is easy to maintain the product during use in the future. By designing the stirring unit in the reactor body, the current low reaction efficiency is overcome. The problem is that the centerline of the rotating shaft in the stirring unit and the centerline of the kettle body are perpendicular to each other, the centerlines of each of the rotating shafts are parallel to each other, and a number of stirring blades are evenly arranged on the outer circumference of the rotating shaft, and the stirring blades There are several mesh holes on the top, which can ensure that the stirring shaft is stably fixed in the kettle body during the stirring process, avoiding the problem of device instability during the reaction process; by including mesh stirring blades in the stirring blades and solid stirring blades The use can realize the full mixing and stirring of the reactants during the stirring process. With the vacuum unit, the air in the reactor body can be evacuated during the reaction process to create a convenient environment for the reaction. Multiple stirring devices are installed to avoid the lower end of the reactor The material is deposited at the lower end, and on the other hand, it can prevent the material from reacting with other impurities, which will affect the quality of the finished product; by setting a temperature control unit in the kettle body, the temperature control cost is low, the structure is simple, and the temperature control method is fast during the synthesis process. The effect is obvious, the price of platinum resistance is low, and it is convenient for maintenance and replacement in later use. The platinum resistance is connected to the power supply through the heating control circuit to realize temperature control, and the control method is fast, which can avoid the problem that the temperature cannot be transmitted in time

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