A method for improving the germination rate of Codonopsis verticillum seeds
A technology of Codonopsis verticillum and germination rate, applied in germination equipment, seed and rhizome treatment, botanical equipment and methods, etc., can solve the problems of strong seed dormancy, restrictions on artificial cultivation of Codonopsis verticillum, low germination rate, etc.
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[0015] Experimental Materials
[0016] Gibberellin (content 75% powder), produced by Shanghai Tongrui Biotechnology Co., Ltd.; ethanol is analytically pure; for the test Codonopsis pilosula seeds.
[0017] Experimental method (in two phases)
[0018] Solution preparation
[0019] Take 7 beakers, wash and dry them, and mark the concentration values. According to the corresponding concentration requirements, weigh 50mg, 100mg, 150mg, 250mg, 350mg, and 450mg of gibberellin, respectively, into a beaker, and add a small amount of ethanol to stir. Finally add water to make up to 500mL.
[0020] The first stage
[0021] The Codonopsis verticillum seed is divided into 63 parts, every part 200, packs with gauze respectively and then loads successively concentration and is respectively 0mg / L (ck), 100mg / L, 200mg / L, 300mg / L, 500mg / L, Soak in a beaker of 700mg / L and 900mg / L gibberellin solution for 12 hours, take it out and rinse it with clean water; take 63 petri dishes, wash and dr...
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