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Microbial preparation for soil remediation

A technology of microbial preparations and composite microbial preparations, which is applied in the field of microbial preparations for soil remediation, can solve the problems of few soil pollution remediation methods, and achieve the effects of improving soil compaction, degrading organic pollutants, and good adsorption

Active Publication Date: 2018-10-09
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes an effective method used by plants called Micrococcus baccatus to treat contamination from landfill sites or other sources. It involves mixing small pieces made up mostly of silicate rocks together with dirt into clay mixtures like bentonite sandwiches. These mixed materials are then placed around litter boxes where they slowly release harmful metals such as lead, mercury, cadmium, chroma, copper, zinc, manganese, nickel, selenium, arsenic, silver, gold, cobalt, tungsten, tantalum, cervical cancer cells, etc., thereby reducing environmental concerns associated therewith.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving environmental quality during construction or other activities where contaminated areas may develop due to excessive use of landfill materials such as plastic pipes used for drainage systems. These compounds contain harmful chemical substances like lead (Pb), mercury(Hg) , arsenite (AsO2), chromate (Cr3+)) etc., causing serious damage when they enter our bodies from rainwater runoff into streams or rivers. Current solutions involve excavation-based cleanup techniques involving removing all hazardous components before disposal, but it cannot effectively remove certain types of polluting agents found within the surrounding ground surface layer without damaging beneficial life forms around them.

Method used

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  • Microbial preparation for soil remediation
  • Microbial preparation for soil remediation
  • Microbial preparation for soil remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the microbial preparation for soil remediation is:

[0023] 1) Preparation of Arthrobacter sp. LHM7719 fermentation broth:

[0024] Arthrobacter sp. LHM7719 was placed in a Erlenmeyer flask equipped with a seed culture base, and the seeds were cultured to the logarithmic phase under the conditions of pH 7.2 and 30°C, and then the inoculation amount was 2% of the medium volume. The strains cultivated to the logarithmic phase were inoculated into LB liquid medium at pH 7.0 and 30° C., shaken at 180 rpm, and cultivated for 48 hours to obtain Arthrobacter sp. LHM7719 fermentation broth.

[0025] 2) Preparation of Rhodopseudomonas palustris fermentation broth:

[0026] The activated Rhodopseudomonas palustris 2-8 (Rhodopseudomonas palustris 2-8) was inoculated into liquid medium A, and the light intensity was 2000 lux, and the temperature was 28-30 ° C under anaerobic culture for 3 days, and then according to 3 % inoculum concentration in liquid m...

Embodiment 2

[0032] The preparation method of the microbial preparation for soil remediation is:

[0033] 1) Preparation of Arthrobacter sp. LHM7719 fermentation broth:

[0034] Arthrobacter sp. LHM7719 was placed in a Erlenmeyer flask equipped with a seed culture base, and the seeds were cultured to the logarithmic phase under the conditions of pH 7.2 and 30°C, and then the inoculation amount was 5% of the medium volume. The strains cultivated to the logarithmic phase were inoculated into LB liquid medium, pH 7.0, 35°C, shaker 150rpm, and cultured for 48 hours to obtain Arthrobacter sp. LHM7719 fermentation broth.

[0035] 2) Preparation of Rhodopseudomonas palustris fermentation broth

[0036] Inoculate the activated Rhodopseudomonas palustris 2-8 (Rhodopseudomonas palustris 2-8) into liquid medium A, and culture it anaerobically for 3 days at a light intensity of 2000 lux and a temperature of 28-30°C, and then follow 2 % inoculum concentration in liquid medium A, anaerobic culture and...

Embodiment 3

[0042] The preparation method of the microbial preparation for soil remediation is:

[0043] 1) Preparation of Arthrobacter sp. LHM7719 fermentation broth:

[0044] Arthrobacter sp. LHM7719 was placed in a Erlenmeyer flask equipped with a seed culture base, and the seeds were cultured to the logarithmic phase under the conditions of pH 7.2 and 30°C, and then the inoculation amount was 3% of the medium volume. The strains cultured to the logarithmic phase were inoculated into LB liquid medium, pH 7.0, at 28°C, shaken at 120 rpm, and cultured for 48 hours to obtain Arthrobacter sp. LHM7719 fermentation broth.

[0045] 2) Preparation of Rhodopseudomonas palustris fermentation broth

[0046] The activated Rhodopseudomonas palustris 2-8 (Rhodopseudomonas palustris 2-8) was inoculated into liquid medium A, and the light intensity was 2000lux, and the temperature was anaerobic culture at 30°C for 3 days, and then according to 5% The inoculum concentration is in the liquid medium A,...

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Abstract

The invention discloses a microbial preparation for soil remediation. The microbial preparation comprises a porous support and a composite microbial agent loaded in the porous support. The porous support comprises diatomite, calcium sulfate and sodium alginate. The composite microbial agent comprises an Arthrobacter sp. LHM7719 microbial agent, a Rhodopseudomonas palustris microbial agent and ACCdeaminase. The Arthrobacter sp. LHM7719 microbial agent is used for the field of soil remediation, and is composited with the Rhodopseudomonas palustris microbial agent and ACC deaminase to obtain a microbial soil remediation agent which can regulate soil pH value and reduce soil hardening, reduce content of heavy metal in soil and degrade organic pollutant in soil.

Description

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Claims

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Application Information

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Owner 江苏世邦生物工程科技有限公司
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