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Total anthocyanin extract of berberis heteropoda and purification and separation method thereof

A technology of Berberis nigra and anthocyanins, which is applied in the field of total anthocyanin extract of Berberis nigra and its purification and separation, can solve the problems of low purity and low color value of the total anthocyanin extract of Berberis nigra, and achieves The effect of stable process, improved purity and color value, and low cost

Inactive Publication Date: 2018-09-25
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides an extract of total anthocyanins of berberis nigrumensis and its purification and separation method, which overcomes the above-mentioned deficiencies in the prior art, and purifies berberis nigrum

Method used

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  • Total anthocyanin extract of berberis heteropoda and purification and separation method thereof
  • Total anthocyanin extract of berberis heteropoda and purification and separation method thereof
  • Total anthocyanin extract of berberis heteropoda and purification and separation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1: The total anthocyanin extract of Barberry black fruit is obtained according to the following method: the first step, at room temperature, after the required amount of Berberis black fruit is pulverized, the slurry is taken, and the acid-containing ethanol solution is used to Extract four times, filter, combine the filtrates and rotary steam to obtain the total anthocyanin concentrate, wherein the ratio of the mass concentration of Berberis nigrum fruit serum to the acid-containing ethanol solution is 1:25; in the second step, use a pH of 1.0 to 5.0 buffer solution to dilute the total anthocyanin concentrate to obtain a total anthocyanin dilution with a pH of 1.0 to 3.0 and a mass concentration of 0.1 mg / mL to 1.5 mg / mL; the third step is to dilute the total anthocyanin dilution at 0.5 The flow rate of mL / min to 3mL / min passes through the pretreated macroporous resin. Every 50mL of total anthocyanin dilution is absorbed by 1g of pretreated macroporous resin....

Embodiment 2

[0040] Embodiment 2: The total anthocyanin extract of Barberry black fruit is obtained according to the following method: the first step, at room temperature, after the required amount of Berberis black fruit is pulverized, the slurry is taken, and the acid-containing ethanol solution Extract four times, filter, combine the filtrates and rotary steam to obtain the total anthocyanin concentrate, wherein the ratio of the mass concentration of Berberis nigrum fruit serum to the acid-containing ethanol solution is 1:25; in the second step, use a pH of 1.0 or 5.0 buffer solution to dilute the total anthocyanin concentrate to obtain a total anthocyanin dilution with a pH of 1.0 or 3.0 and a mass concentration of 0.1 mg / mL or 1.5 mg / mL; the third step is to dilute the total anthocyanin dilution to 0.5 The flow rate of mL / min or 3mL / min passes through the pretreated macroporous resin, and every 50mL of the total anthocyanin dilution is absorbed by 1g of the pretreated macroporous resin...

Embodiment 3

[0041] Embodiment 3: as the optimization of above-mentioned embodiment, the volume percent concentration of the acid-containing ethanol solution used during extraction is 60% to 80%, the contained acid of the acid-containing ethanol solution is hydrochloric acid, and the volume percent concentration of hydrochloric acid is 0.8%, Each extraction time is 20min to 30min; or / and, the macroporous resin is NKA-9 macroporous resin, X-5 macroporous resin, AB-8 macroporous resin, HPD-100 macroporous resin, HPD-300 macroporous resin Resin, one of XDA-6 macroporous resin, XDA-7A macroporous resin, D101 macroporous resin, XAD-16 macroporous resin, XAD-7HP macroporous resin.

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Abstract

The invention relates to the technical field of anthocyanin extraction of berberis heteropoda, and particularly to a total anthocyanin extract of berberis heteropoda and a purification and separationmethod thereof. The total anthocyanin extract of the berberis heteropoda is obtained according to the following method: crushing fruits of the berberis heteropoda to obtain syrup, extracting with an acid-containing ethanol solution, filtering, combining the filtrate and performing rotary evaporateion to obtain a total anthocyanin concentrated solution, diluting the total anthocyanin concentrated solution with a buffer solution to obtain a diluted total anthocyanin solution, performing macroporous resin adsorption on the diluted total anthocyanin solution, washing the resin with distilled waterand pumping, adding an ethanol solution into the pumped resin for elution, performing concentration and drying to obtain the total anthocyanin extract of the berberis heteropoda. According to the total anthocyanin extract of the berberis heteropoda and the purification and separation method thereof, the anthocyanin of the berberis heteropoda is purified by adopting an XDA-7A type macroporous adsorption resin, the characteristics of simple operation, low cost and stable technology are realized, and the purity and color value of the obtained total anthocyanin extract of the berberis heteropodaare improved.

Description

technical field [0001] The invention relates to the technical field of barberry anthocyanin extraction, and relates to an extract of total anthocyanins from berberis nigrum and a purification and separation method thereof. Background technique [0002] Berberis berberis (Berberisheteropoda Schrenk.) is a shrub of Berberidaceae, mainly distributed in Xinjiang Uygur Autonomous Region, with rich resources. It is a medicinal and edible plant, and its mature fruit contains rich anthocyanins. Anthocyanins are flavonoid polyphenol compounds formed by the combination of anthocyanins and sugars through glycosidic bonds. They have various physiological functions such as anti-free radicals, prevention of cardiovascular diseases, and anti-tumors. They have broad applications in the fields of medicine, food, and cosmetics. Application prospect. [0003] The separation and purification methods of anthocyanins include macroporous adsorption chromatography, polyamide chromatography, gel ch...

Claims

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Application Information

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IPC IPC(8): A61K36/29A61K131/00
CPCA61K36/29A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 李建光迪娜·吐尔洪阳莹刘倩芸侯亚申
Owner XINJIANG MEDICAL UNIV
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