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A kind of sperm gradient centrifugal liquid and preparation method thereof

A gradient centrifugation and sperm technology, applied in the field of assisted reproduction, can solve the problems of inability to reduce physical damage, large pH fluctuations, and limited pH buffering capacity, and achieve the effects of reducing sperm damage, stimulating sperm vitality, and strong buffering capacity

Active Publication Date: 2021-08-31
成都艾伟孚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The buffer used in most sperm gradient centrifugation fluids is HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). The pH buffering capacity of a single buffer system is limited, and the pH fluctuation is large; on the other hand, generally Sperm gradient centrifugation fluid relies on the addition of glucose to enable sperm capacitation, but does not reduce the physical damage to sperm caused by centrifugation

Method used

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  • A kind of sperm gradient centrifugal liquid and preparation method thereof
  • A kind of sperm gradient centrifugal liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Sperm Gradient Centrifugation Solution 3

[0058] 1) Using water as the base, prepare the base liquid according to the basic formula;

[0059] 2) Add C component 10mmol / L and D component 4.10mmol / L, divide the solution into 2 equal parts, marked as solution 1 and solution 2;

[0060] 3) Add component A to solution 1 to make the final concentration reach 40%, which is marked as a1, and add component A to solution 2 to make the final concentration reach 80%, which is marked as a2;

[0061] 4) a1 and a2 were sterilized by filter membrane respectively.

Embodiment 2

[0062] Example 2 Sperm Gradient Centrifugation Solution 4

[0063] 1) Using water as the base, prepare the base liquid according to the basic formula;

[0064] 2) Add C component 10mmol / L and D component 4.10mmol / L, add B component 30mg / L to divide the solution into 2 equal parts, marked as solution 1 and solution 2,

[0065] 3) Add component A to solution 1 to make the final concentration reach 40%, which is marked as a1, and add component A to solution 2 to make the final concentration reach 80%, which is marked as a2;

[0066] 4) a1 and a2 were sterilized by filter membrane respectively.

Embodiment 3

[0067] Example 3 Sperm Gradient Centrifugation Solution 5

[0068] 1) Using water as the base, prepare the base liquid according to the basic formula;

[0069] 2) Add C component 10mmol / L and D component 4.10mmol / L, add B component 100mg / L to divide the solution into 2 equal parts, marked as solution 1 and solution 2,

[0070] 3) Add component A to solution 1 to make the final concentration reach 40%, which is marked as a1, and add component A to solution 2 to make the final concentration reach 80%, which is marked as a2;

[0071] 4) a1 and a2 were sterilized by filter membrane respectively.

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Abstract

The invention belongs to the field of assisted reproduction, and in particular relates to a sperm gradient centrifugal liquid and a preparation method thereof. The sperm gradient centrifugation liquid of the present invention adopts the HEPES+MOPS double buffer system to regulate the pH of the sperm gradient centrifugation liquid, and the pH is more stable; the key raw material lecithin is added to the sperm gradient centrifugation liquid to stimulate sperm vitality and repair and reduce sperm damage. The sperms screened by the sperm gradient centrifugation fluid of the present invention have strong vigor and higher normal morphology rate, and are fully prepared for subsequent in vitro fertilization.

Description

technical field [0001] The invention belongs to the field of assisted reproduction, and in particular relates to a sperm gradient centrifugal liquid and a preparation method thereof. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) refers to the technology in which human sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is abbreviated as IVF in English. A large number of people are unable to conceive naturally due to physical conditions, which requires in vitro fertilization-embryo transfer, commonly known as test-tube baby technology, for reproduction. According to a survey data, the infertility rate in my country has climbed from 2.5%-3% 20 years ago to 12.5%-15%. The number of newborns born in my country is about 16 million per year. Based on the infertility rate of 12.5%-15%, in theory, 2-2.4 million newborns cannot be born each year due to infertility. With the implementation of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/076
CPCC12N5/061C12N2509/10
Inventor 余裕炉米乐刘洪君严飞戴甄
Owner 成都艾伟孚生物科技有限公司
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