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A kind of incubation method of dormant eggs of black pearl killifish

A technology for dormant eggs and pearls, applied in the field of hatching, can solve the problems of low survival rate of fry cultivation, low spawning quantity of seed fish, low fertilization rate, etc., and achieve the effects of precise hatching time node, shortening hatching time, and speeding up the development process.

Active Publication Date: 2021-05-28
ZHEJIANG NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in the market industrialization of killifish, such as low egg production, low fertilization rate, low egg hatching rate, and low survival rate of fry cultivation, which restrict the development of ornamental killifish market and industrial development
[0003] The Chinese patent with the application number 201610259750.X discloses a method for efficient reproduction and hatching of medaka fish, and provides a method with strong controllability and high breeding efficiency, which can realize efficient reproduction and hatching of medaka fish. The method is aimed at the medaka, which belongs to different families and genera from the oviparous medaka, the black pearl medaka. After the medaka eggs are mature, the female fish will produce them, but they will not leave the mother body, but pass through The sticky silk hangs outside the mother's cloaca, and there is no dormant period, so hatching is relatively simple. However, for the hatching of annual killifish, the fertilized eggs must go dormant out of water for 2-3 months. Even for the same batch of fertilized eggs, the hatching time Not fully consistent and therefore very unsuitable for commercialization

Method used

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  • A kind of incubation method of dormant eggs of black pearl killifish

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Experimental program
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Effect test

Embodiment 1

[0028] Preparation of hatching water: add 10wt% peat soil extract and 20ppm glutaraldehyde to the water, keep the water temperature at 27°C, and circulate and filter it with a biochemical cotton filter for 3 days.

[0029] Seal the dormant eggs in a sealed bag wrapped with biochemical cotton and plastic wrap. After the fish eggs have been dormant for 50 days at 25°C, check the development of the eggs every 3 days; Higher than 2°C. On the 15th day after all dormant eggs have golden eye circles, take out the dormant eggs and place them in a metal screen. Spray the eggs with hatching water. The spray lasts for 5 minutes, and then sprays for another 5 minutes every 20 minutes; the spraying ends 20 minutes Finally, the metal screen is placed in a hatching tank with a specification of 50cm x 30cm x 25cm in length, width and height. 15cm of hatching water is added to the hatching tank. The mouth of the screen is inclined to one side, so that the unswimmed larvae are free from the scr...

Embodiment 2

[0031] Preparation of hatching water: add 9wt% peat soil leaching solution and 30ppm glutaraldehyde to the water, the water temperature is 26.5°C, and circulate and filter with a biochemical cotton filter for 3 days.

[0032] Seal the dormant eggs in a sealed bag wrapped with biochemical cotton and plastic wrap. After the eggs have been dormant at 27°C for 50 days, check the development of the eggs every 2 days; 1°C higher, and on the 14th day after all dormant eggs have golden eye circles, take out the dormant eggs and place them in a metal screen, and spray the eggs with hatching water for 4.5 minutes, and then spray for 4.5 minutes every 22 minutes; After 22 minutes, place the metal screen in an incubation tank with dimensions of 55cm x 35cm x 20cm in length, width and height. Add 13cm of hatching water to the incubation tank, and the top of the metal screen is submerged 2cm under the water. After 6.5 hours, some eggs will hatch out, tilt the opening of the sieve to one sid...

Embodiment 3

[0034] Preparation of hatching water: add 11wt% peat soil leaching solution and 25ppm glutaraldehyde to the water, the water temperature is 27.5°C, and circulate and filter with a biochemical cotton filter for 3 days.

[0035]Seal the dormant eggs in a sealed bag wrapped with biochemical cotton and plastic wrap. After the eggs are dormant at 23°C for 50 days, check the development of the eggs every 3 days; Higher than 2°C. On the 16th day after all dormant eggs have golden eye circles, take out the dormant eggs and place them in a metal screen, spray the eggs with hatching water for 5.5 minutes, and then spray for 5.5 minutes every 18 minutes; spray After 18 minutes, place the metal screen in an incubation tank with dimensions of 45cm x 25cm x 30cm in length, width and height. Add 17cm of hatching water to the incubation tank. The top of the metal screen is submerged 4cm below the water. After 5.5 hours, some eggs will hatch. out, tilt the sieve opening to one side to free the...

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Abstract

The invention relates to a hatching method, and discloses a method for hatching dormant eggs of black pearl killifish. The method comprises the following steps: 1) preservation of dormant eggs; 2) pretreatment of dormant eggs; 3) hatching in water; 4) unhatched Two eggs hatch. The invention saves fertilized eggs in a soilless way, can accurately judge the development status of fish eggs, makes the hatching time node more accurate, accelerates the development process by heating up and spraying drugs, and increases the hatching rate by 30% compared with the traditional hatching method. The number of hatching times is only 2 times, the hatching time is greatly shortened, and there is no need to manually pick eggs, which greatly saves manpower. It is a method for hatching dormant eggs of black pearl killifish suitable for commercial applications.

Description

technical field [0001] The invention relates to a hatching method, in particular to a hatching method for dormant eggs of black pearl killifish. Background technique [0002] The black pearl killifish is an annual oviparous killifish in South America. It has a very attractive body color. It can be said to be one of the few fish species in freshwater ornamental fish that can be compared with sea coral reef fish. In addition to their small size, they are lively With an active personality, it is a perfect match in a planted tank. In addition, most oviparous killifish have wide adaptability to water quality and are easy to raise. These characteristics make them one of the most popular tropical ornamental fish species at home and abroad. Especially the fertilized eggs of oviparous killifish have a characteristic that they can be dormant for a certain period of time. Therefore, the fish eggs can be sold on the market. However, the reproductive behavior of oviparous killifish is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/17
CPCA01K61/17Y02A40/81
Inventor 施振宁
Owner ZHEJIANG NORMAL UNIVERSITY
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