A kind of sex pheromone for preventing and controlling vermilion moth and preparation method of lure core
A technology of vermilion moth and pheromone, which is applied in the field of insect sex pheromone preparation, can solve the problems of polluting the environment, threatening human health, killing natural enemies by mistake, etc.
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Embodiment 1
[0016] Extraction of sex pheromone of Vermillion moth
[0017] The headspace adsorption method and gonadal extraction method were used to extract the sex pheromone of the unmated females of the Vermillion moth at different ages and entering the photoperiod at different times. The detection was carried out by gas chromatography-mass spectrometry and the insect antenna potential measurement System testing showed that the sex information of Vermillion Moth is composed of cis-9-hexadecenal and (Z,Z,Z)-9,12,15-octadecenal.
Embodiment 2
[0019] Preparation of the sex pheromone lure of P. versicolor
[0020] At room temperature, first mix 10 microliters of cis-9-hexadecenal and 10 microliters of (Z,Z,Z)-9,12,15-octadecenal to 980 microliters In the n-hexane solution, a mixture of two sex pheromones with a mass ratio of 1:1 is formed; secondly, the mixed liquid containing the sex pheromone of the vermillion moth is added dropwise to the cotton sliver lure. After the n-hexane in the sex pheromone mixture is volatilized, a lure containing vermillion moth attractant is prepared; finally, the prepared lure is sealed in aluminum foil, and stored at -20°C.
Embodiment 3
[0022] The trapping effect of the sex pheromone of Vermillion moth
[0023] Set the following 8 kinds of lures:
[0024] A. Add 10 microliters of cis-9-hexadecenal to 990 microliters of n-hexane solution, and add the mixed liquid dropwise to the tampon lure core. After the n-hexane volatilizes, it will produce vermilion hair The lure of the moth attractant;
[0025] B. Add 10 microliters of (Z,Z,Z)-9,12,15-octadecatrienal to 990 microliters of n-hexane solution, and add the mixture dropwise to the tampon lure. After the volatilization of n-hexane, the lure core containing vermillion moth attractant is prepared;
[0026] C. Add 10 microliters of trans-9-hexadecenal to 990 microliters of n-hexane solution, and add the mixed liquid dropwise to the tampon lure core. After the n-hexane volatilizes, the product contains vermilion hair The lure of the moth attractant;
[0027] D, add 10 microliters of cis-9-hexadecenal and 10 microliters of (Z,Z,Z)-9,12,15-octadecenal to 980 microliters of ...
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