A kind of broad bean rhizobia strain blgs19-2 and its application

Abstract

A faba bean rhizobium strain Blgs19‑2 and application thereof, the strain is isolated and purified from fresh faba bean nodules, and belongs to Rhizobium anhuiense strains. The strain was deposited in the Chinese Type Culture Collection Center of Wuhan University on October 23, 2017, and the preservation number is: CCTCC NO: M2017614. The rhizobium strain Blgs19-2 of the present invention is an excellent broad-spectrum faba bean rhizobia with strong symbiotic nitrogen fixation ability, wide adaptability to Sichuan broad bean varieties, secretion of IAA, ability to dissolve inorganic phosphorus and organic phosphorus, and strong stress resistance The strain has a good matching affinity with the main broad bean varieties in Sichuan. In the production of different varieties of broad beans in Sichuan, no nitrogen fertilizer and inoculation with Blgs19-2 increased the yield of broad beans by more than 37.8%, and the difference from the control without inoculation reached a significant level.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a broad bean rhizobium strain Blgs19-2 and its application. Background technique [0002] China is the largest broad bean producer, with dry broad bean production accounting for 35% of the world (FAO2014). It is an important agricultural product resource for my country's traditional foreign trade, and its export volume ranks second among the country's miscellaneous grains (Liu Yujiao et al., 2002). The sown area of ​​broad beans in Sichuan Province is about 300,000 hectares, second only to Yunnan, with a total output of 600 million kilograms (Yang Wuyun, 2003). Douban in Pixian County, Sichuan, which uses broad beans as raw materials, is well-known throughout the country and is in great demand. The annual demand for broad beans reaches 120,000 tons, but now it mainly relies on imports from other places. In addition to harvesting dry broad beans, fresh broad beans can also be harves...

AI Technical Summary

Problems solved by technology

Shi Shangli (2007) conducted research on more than 730 strains of Medicago rhizobia, and only 10 strains of rhizobia with strong ability to secrete IAA were screened from these 29 strains, and the phosphorus-dissolving ability of these 29 strains was measured, and it was found that all The strains did not have the ability to dissolve inorganic phosphorus. The 29 Rhizobia meliloti could dissolve organic phosphorus, but the phosphorus-dissolving ability was quite different. Only 8 strains had strong ability to dissolve organic phosphorus, and the rest had weak or weak ability to dissolve organic phosphorus. Organophosphate capacity

[0006] At present, there are no relevant reports on the screening application of high-efficiency rhizobia in broad bean except Qinghai, Gansu (Diao Zhimin, 2002; Han Mei et al., 2010; Wang Wenli et al., 2010) and Hebei (Wang Rui, 2016). other reports

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