Novel fermented soybean meal as well as preparation process and application thereof
A preparation process and technology for fermenting soybean meal, applied in the direction of bacteria, application, food processing, etc. used in food preparation, can solve the problems of uneven quality, single selection of bacteria, poor process controllability, etc., and achieve uniform quality, Simple preparation process and the effect of improving feed intake
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[0028] The preparation process of the novel fermented soybean meal provided by the embodiment of the present invention mainly includes the following steps: preparing the seed solution of the bacteria, preparing the bacteria solution after expanding the culture, mixing the bacteria solution and the soybean meal and fermenting.
[0029] The strains used for preparing the strain seed solution include Hansenula anomalies, Bacillus subtilis and Lactobacillus acidophilus. In the embodiment of the present invention, Hansenula anomalius is used instead of the commonly used Saccharomyces cerevisiae for fermentation to produce esters and produce aroma, which is thicker and thicker than Saccharomyces cerevisiae fermentation, thereby improving the food-attractiveness of the feed. In addition, the above strains contain not only Hansenula anomaly and Bacillus subtilis which ferment aerobically, but also Lactobacillus acidophilus which ferment anaerobically. It is beneficial to improve the n...
Embodiment 1
[0062] Inoculate Hansenula abnormalis slant in YPD liquid medium, culture at 30°C and 200r / min for 20 hours, divide into shake flasks, and prepare 1.8L Hansenula abnormalis seed solution. Bacillus subtilis slant was inoculated in LB liquid medium, cultured at 37°C and 200r / min for 20h, divided into shake flasks, and 1.8L Bacillus subtilis seed solution was prepared. Lactobacillus acidophilus slant was inoculated in MRS liquid medium and cultured statically for 48 hours to prepare 1.8L Lactobacillus acidophilus seed solution.
[0063] The seed liquid of Hansenula anomaly, Bacillus subtilis and Lactobacillus acidophilus were respectively inoculated into three different 3-ton fermenters according to the inoculum amount of 0.5vt‰, and the loading amount was 60vt%. Among them, the Hansenula anomaly seed liquid and the Bacillus subtilis seed liquid were cultured for 15 hours, and the seed liquid of Lactobacillus acidophilus was cultured for 24 hours. The number of live bacteria in ...
Embodiment 2
[0072] Inoculate Hansenula abnormalis slant in YPD liquid medium, culture at 30°C and 200r / min for 24 hours, divide into shake flasks, and prepare 1.8L Hansenula abnormalis seed solution. Bacillus subtilis slant was inoculated in LB liquid medium, cultured at 37°C and 200r / min for 24 hours, divided into shake flasks, and 1.8L Bacillus subtilis seed solution was prepared. Lactobacillus acidophilus slant was inoculated in MRS liquid medium and cultured statically for 48 hours to prepare 1.8L Lactobacillus acidophilus seed solution.
[0073] The seed liquid of Hansenula anomalius, Bacillus subtilis and Lactobacillus acidophilus were inoculated into three different 3-ton fermenters according to the inoculation amount of 1.5vt‰, and the loading amount was 60vt%. Among them, the Hansenula anomaly seed liquid and the Bacillus subtilis seed liquid were cultured for 18 hours, and the seed liquid of Lactobacillus acidophilus was cultured for 28 hours. The number of live bacteria in Han...
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