Compound amplification kit for simultaneously detecting female skin and hair SNP (Single Nucleotide Polymorphism) genetic polymorphism site and application thereof
A technique of genetic polymorphism and multiple amplification, which is applied in the field of multiple amplification kits for simultaneous detection of female skin and hair SNP genetic polymorphism sites, can solve the problems of few, high cost of one-time detection, and high detection position. wait for less
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Embodiment 1
[0097] A multiplex amplification kit for simultaneously detecting female skin and hair SNP genetic polymorphism sites, the kit contains primers for 6 SNP loci, and the 6 SNP loci are: MC1R (rs1805007), NQO1(rs1800566), AQP3(rs17553719), FGFR2(rs4752566), EDAR(rs3827760), MMP1(rs1799750); the rs number in parentheses indicates the number of the site in the dbSNP database.
[0098]The primers include shared upstream primers and specific downstream primers for each SNP locus, and the specific downstream primers introduce mismatched bases at the 2nd, 3rd or 4th position of the 3' end.
[0099] The primers and their sequences are as follows:
[0100] The shared upstream primers used to detect locus MC1R rs1805007C / T / G / A are:
[0101] SEQ1: 5'-ATGAGCACCAGCATGGCTC-3';
[0102] The specific primer used to detect site MC1R rs1805007C is one of the following nucleotide sequences:
[0103] SEQ2: 5'-TCTCCATCTTCTACGCA T TGC-3';
[0104] SEQ3: 5'-TCTCCATCTTCTACGCAC G GC-3';
[0105] ...
Embodiment 2
[0179] 1. Collection of test materials: The test materials were oral swabs of volunteers, a total of 6 copies.
[0180] 2. Template DNA extraction: The template DNA was extracted from the oral swabs of 6 volunteers by Chelex-100 method.
[0181] 3. PCR amplification:
[0182] (1), the multiple amplification detection primers used are:
[0183] MC1Rrs1805007
SEQ1: 5'-ATGAGCACCAGCATGGCTC-3'
C
SEQ4: 5'-TCTCCATCTTCTACGCACT T C-3'
T
SEQ5: 5'-atttCATCTCCATCTTCTACGCA T TGT-3'
G
SEQ9: 5'-atttcttTCTCCATCTTCTACGCAC G GG-3'
A
SEQ12: 5'-atttGGCATCTCCATCTTCTACGCACG A T-3'
NQO1rs1800566
SEQ14: 5'-GCATGGAAGCTGTTGACTTAC-3'
C
SEQ16: 5'-CAATGCTATATGTCAGTT A CGG-3'
T
SEQ20: 5'-atttCAATGCTATATGTCAGTTG A TA-3'
A
SEQ22: 5'-atttattCAATGCTATATGTCAGTTG T GT-3'
AQP3rs17553719
SEQ24: 5'-AGAGTTTGGGGAGTGCATCG-3'
T
SEQ27: 5'-ATCCGCTGCTCGCTGCT C GA-3'
G
SEQ29: 5'-atttA...
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