Casset based on solid-phase type quick blood grouping and grouping method

Abstract

The invention relates to a casset based on solid-phase type quick blood grouping and a grouping method. The casset comprises a casset body, a sample groove, an antibody groove and a gold mark groove,wherein the sample groove is arranged in the middle part of the casset body; the antibody groove is arranged at one side of the sample groove; the gold mark groove is arranged at the other side of thesample groove; a monoclonal antibody is arranged in the antibody groove; a gold mark erythrocyte membrane antigen is arranged in the gold mark groove; an upper water absorbing paper is arranged between the sample groove and the antibody groove; a blood filtering membrane is arranged between the sample groove and the gold mark groove; a lower water absorbing paper is arranged under the blood filtering membrane. The casset has the advantages that the anti-A, anti-B and anti-D specific monoclonal antibodies are used as positive typing reagents of A, B and O blood groups, the A, B and O blood group antigens (gold mark antigens) marked with the specific erythrocyte membranes are used as reverse typing immune colloidal gold reagents, and the positive and reverse typing reagents are respectivelyarranged on the casset body; the operation is simple and quick, the carrying is convenient, the accuracy is high, the casset is suitable for the condition that the positive and reverse typing reagents of the blood group typing reagent are simultaneously placed into a blasting ball at mobile blood collection sites and during emergency blood transfusion.

Description

technical field [0001] The invention relates to a detection board and an identification method for rapid blood type identification based on a solid-phase method. Background technique [0002] Compared with traditional wars, modern wars are more sudden, uncertain and tragic. Due to the continuous development and widespread application of new types of highly lethal weapons, the number of critically injured patients who urgently need blood transfusions for medical treatment has increased dramatically. At the same time, in order to ensure the effective transfusion of various blood products and the safety of blood use in wartime, it is necessary to strictly abide by the standard operating procedures for various tests such as blood type identification and cross-matching before blood transfusion. It is very difficult to guarantee that if the blood type identification and cross-matching are inaccurate, the wounded may be mistakenly transfused with heterotyped blood, resulting in se...

AI Technical Summary

Problems solved by technology

Through the analysis of previous war data at home and abroad, combined with the multiple medical support maneuvers organized by our army and natural disaster rescues such as the Wenchuan Earthquake, we have gained inspiration: At present, the commonly used blood type identification methods in clinical practice include test tube agglutination method and microcolumn gel method , the required detection reagents require storage in a low-temperature refrigerator, the experimental operation requires a centrifuge, and both require power supply, and the latter takes a long time to identify, and also requires special instruments and reagents, and the price is high, neither of which is suitable for emergencies case for field use

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