Detection kit for preeclampsia of pregnant women

A detection kit and a technology for preeclampsia, applied in the field of disease detection, can solve the problems of ratio influence, influence on accurate treatment, influence on the accuracy of the onset risk of eclampsia, etc., achieving simple application, predicting onset risk, and being suitable for large-scale The effect of promoting the application

Inactive Publication Date: 2018-04-06
江苏龙维生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, sFlt-1 and PIGF are two indicators that are closely related but relatively independent; the existing sFlt-1 detection kit and PIGF detection kit are two independent products that detect the two indicators separately. There will be differences in processing, sample loading, etc., which will affect the ratio between the two, and then affect the accuracy of predicting the risk of eclampsia in pregnant women, and further affect the next step of accurate treatment

Method used

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  • Detection kit for preeclampsia of pregnant women
  • Detection kit for preeclampsia of pregnant women
  • Detection kit for preeclampsia of pregnant women

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1 fluorescent labeling

[0037] Cleaning: Put fluorescent microspheres (Bangs Lab, product number: 11233) into a centrifuge tube, add 0.1M MES (morpholineethanesulfonic acid, pH 5.0) buffer and mix well, and centrifuge at 13000rpm, 15min, 4°C, discard The supernatant was resuspended with 0.1M MES (pH 5.0) buffer for use. Activation and cleaning: the activator EDC (1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride), NHS (1-hydroxypyrroline-2,5-dione) and Fluorescent microspheres are activated according to the mass ratio of 2:1:2, the specific operation is as follows:

[0038] Weigh EDC and NHS and add them to 0.1M MES (pH 5.0) buffer solution to dissolve, quickly take an appropriate amount into the washed fluorescent microspheres, seal them with a parafilm and place them on a 200rpm shaker at room temperature for 30min, take them out at 13000rpm for 30min , centrifuged at 4°C to remove the supernatant, resuspended with an equal amount of 0.1M MES (pH 6...

Embodiment 2

[0045] Spray point of embodiment 2 bonding pad

[0046] The method of spraying the antibody (anti-human placental growth factor) binding pad for PIGF diagnosis is as follows: Fluorescence-labeled antibody solution dilution: the fluorescent-labeled antibody conjugate prepared above was diluted 4 times with fluorescent-labeled antibody resuspension; set point Membrane instrument, turn on the power of the film spotting instrument, set the spraying program, the spraying volume is 8ul / cm; the No. 1 pipe is the spraying channel; the film spotting instrument initialization: put the No. 1 pipe in the fluorescently labeled antibody resuspension solution , select the initialization program, and initialize 6 cycles; spray point: place the bonding pad on the spotting device at a fixed position, press the "GO" key on the control panel to start spraying, remove it after pointing, and check that the spraying point is good Conjugation pads, the fluorescently labeled antibody strips sprayed ev...

Embodiment 3

[0048] The preparation of embodiment 3 nitrocellulose membranes

[0049] Antibody (anti-human placenta growth factor) nitrocellulose membrane preparation method for PIGF diagnosis is as follows: take goat anti-human PIGF polyclonal antibody (Sigma, goat anti-human placenta growth factor polyclonal antibody, 0.1ml / bottle) 500ug, add to In a 5ml graduated centrifuge tube, dilute the antibody to 1ml, and mark the container with a T mark. Take 25ul of goat anti-mouse IgG antibody (Hangzhou Longji Biotechnology, goat anti-mouse IgG, 10mg / bottle), add it to a 5ml graduated centrifuge tube, dilute the antibody to 1ml, and mark the container with a C mark. Set the film spotting instrument, turn on the power supply of the film spotting instrument, set the spray point program, and the spray point volume is 1ul / cm; the No. 1 pipeline is the detection channel with spray points, and the No. 2 pipeline is the control channel with spray point points; the film spotting instrument Initializat...

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Abstract

The invention relates to a detection kit for preeclampsia of pregnant women. The kit comprises PIGF and a sFlt-1 immunochromatography detection test strip or a PIGF immunochromatography detection teststrip and an sFlt-1 immunochromatography detection test strip, relatively preferably, a PIGF detecting immunolabelling antibody and a PIGF detecting antibody are separately arranged on a combining pad and a nitrocellulose membrane of the PIGF immunochromatography detection test strip; an sFlt-1 detecting immunolabelling antibody and an sFlt-1 detection antibody are separately arranged on a combining pad and a nitrocellulose membrane of the sFlt-1 immunochromatography detection test strip; or, the immunolabelling antibodies and the antibodies are separately coated to the combining pads and thenitrocellulose membranes of the PIGF and sFlt-1 immunochromatography detection test strips. According to the kit provided by the invention, PIGF and sFlt-1 can be detected at the same time by sampling once, so that the kit is simple to apply, and can acquire the ratio of the two accurately to further predict the onset risk of preeclampsia of pregnant women accurately, thereby providing an effective basis for accurate treatment. The kit is suitable for being popularized and applied on a large scale.

Description

technical field [0001] The invention relates to the technical field of disease detection, in particular to the technical field of disease detection kits, in particular to a detection kit for preeclampsia in pregnant women. Background technique [0002] Eclampsia is one of the main causes of maternal mortality. The global incidence is about 3-5%, and the incidence in developing countries is close to 10%. The harm of eclampsia to pregnant women includes convulsions, placental abruption, disseminated intravascular coagulation, renal failure, pulmonary edema, cerebral hemorrhage, cerebral edema, thromboembolism and death; at the same time, the harm to the fetus includes intrauterine dysplasia, intrauterine Growth restriction, premature birth and stillbirth. [0003] In recent years, theoretical research on placental ischemia believes that soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PIGF) in blood circulation are two important factors that cause and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/573G01N33/68
Inventor 韦彦余王军峰
Owner 江苏龙维生物科技有限公司
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