Application of tomato gene slsaur58 in regulating tomato growth and drought resistance
A drought resistance and tomato technology, applied in the application field of tomato gene SlSAUR58 in regulating tomato growth and drought resistance, can solve the problems that functional research has not been involved, and the effect is unclear, so as to increase the biological yield of plants, improve drought resistance, The effect of promoting the vegetative growth process
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Embodiment 1
[0021] Example 1 Spatiotemporal expression of SlSAUR58 gene in different tissues and organs of tomato
[0022] Using the cDNA of roots, stems, leaves, flowers and fruits of the tomato variety "Micro-Tom" as a template, the spatiotemporal expression of SlSAUR58 in different parts of tomato was detected, SlSAUR58-S:
[0023] CCAATACTGGAAGACACCC (SEQ ID NO:4); SlSAUR58-A:
[0024] CACAAAACACCACCATCACC (SEQ ID NO:5). Such as figure 1 The expression of SlSAUR58 shown is the lowest in tomato roots; the expression in leaves is the highest, which can reach more than 30 times the expression in roots. Further detection of the expression of SlSAUR58 in tomato flowers at six stages revealed that the expression of SlSAUR58 was gradually increasing with the development of flowers.
Embodiment 2
[0025] Example 2 Response pattern of SlSAUR58 to drought stress
[0026] The tomato "Micro-Tom" plants that have grown to about 4 weeks are placed in an incubator with a humidity of 40%, and are subjected to water-deficient treatment. On the 0d, 3d, 6d and 9d after the water-deficient treatment, the same leaf position Leaves were collected for quantitative analysis. QRT-PCR analysis found that in the leaves of drought-treated tomato plants ( Figure 7 ), the expression level of SlSAUR58 increased significantly, and the expression level of SlSAUR58 reached the highest value 6 days after the drought treatment, which was about 6 times that of the control. Drought stress can obviously induce the expression of SlSAUR58.
Embodiment 3
[0027] The promoter analysis of embodiment 3 SlSAUR58 gene
[0028]In this study, the promoter sequence 2000bp upstream of SlSAUR58 was isolated from the tomato SGN database, and the cis-acting elements of this promoter were predicted on the PlantCare website. element), ABA (ABRE) and GA (GARE-motif) response elements.
[0029] In this study, specific primers were further designed: P58-S (SEQ ID NO: 6) and (P58-A SEQ ID NO: 7), amplified and isolated the promoter sequence of 1302 bp upstream of the SlSAUR58 gene (including the above hormone-related elements) ( figure 2 A), constructed the promoter vector of SlSAUR58, namely pSlSAUR58::GUS-GFP-PBGWFS7.0, and found that the 1300bp promoter sequence upstream of the gene of SlSAUR58 can activate the expression of green fluorescent protein, indicating that the isolated promoter sequence of SlSAUR58 with activation activity such as figure 2 B and C. The constructed vector was transformed into Arabidopsis thaliana by flower dip...
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