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A kind of method for preparing protein peptide by protease enzymatic hydrolysis

A protein peptide and protease technology, applied in the field of biochemistry, can solve problems such as side reactions, high production costs, losses, etc., and achieve the effect of enhancing enzymatic hydrolysis and reasonable component configuration

Active Publication Date: 2021-06-08
舟山市常青海洋食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the shortcomings of this method also cause the production cost to be too high, and it is difficult to achieve large-scale production. In the process of amino acid synthesis, organic solvents will be used to react, which will cause certain damage to the human body. At the same time, there will be Many side reactions occur, and many by-products are produced at the same time, causing unnecessary losses, which makes the method of chemically synthesizing polypeptides hindered to varying degrees.

Method used

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  • A kind of method for preparing protein peptide by protease enzymatic hydrolysis
  • A kind of method for preparing protein peptide by protease enzymatic hydrolysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0023] Table 1: Conditions for preparing protein peptides by enzymatic hydrolysis of flavor protease in Example 1-5 of the present invention

[0024]

Embodiment 1

[0026] Take the substrate protein with a concentration of 4%, adjust the pH to 6.5 with 10mol / L sodium hydroxide or hydrochloric acid solution, and when the temperature is 45°C, add flavor protease, vitamin B1 and Sichuan Dome, stirred on a magnetic stirrer for 1.5h to carry out enzymatic hydrolysis reaction to obtain protein peptides.

Embodiment 2

[0028] Take the substrate protein with a concentration of 4.25%, adjust the pH to 6.7 with 10mol / L sodium hydroxide or hydrochloric acid solution, and when the temperature is 48°C, add flavor protease, vitamin B1 and Sichuan Dome, stirred on a magnetic stirrer for 1.8h to carry out enzymatic hydrolysis reaction to obtain protein peptides.

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Abstract

A method for preparing protein peptides by enzymatic hydrolysis of proteases, involving the field of biochemistry, comprising the following steps: firstly adjusting the pH of a substrate protein to 6.5-7.5, then adding flavor protease, vitamin B1, and cava, and stirring to carry out enzymatic hydrolysis reaction to obtain protein Peptide: In the method for preparing protein peptide by enzymatic hydrolysis of protease of the present invention, flavor protease is selected, and vitamin B1 and vitamin B1 are added, which can activate the enzyme activity of flavor protease and enhance the enzymatic hydrolysis of protein.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a protease hydrolysis method for preparing protein peptides. Background technique [0002] Protein peptide is a kind of biologically active substance, which is a mixed oligopeptide with certain biological functions obtained by proteolysis of protein or microbial fermentation. From a chemical point of view, protein peptide is a general term for different peptides ranging from dipeptides to complex linear and circular structures composed of 20 natural amino acids in proteins in different compositions and arrangements. Protein peptides have a variety of body metabolism and physiological regulation functions for digestion and absorption. It also has the functions of lowering blood sugar, lowering blood fat, relaxing blood vessels, promoting bone anabolism to maintain its normal structure and function, promoting growth, promoting cell differentiation, and wound repair. [0003] The prepa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 丁璞
Owner 舟山市常青海洋食品有限公司
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