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Anti-CXCL9, anti-CXCL10, anti-CXCL11, anti-CXCL13, anti-CXCR3 and anti-CXCR5 reagents used for inflammatory diseases

A technology for inflammatory diseases and CCR5, applied in anti-inflammatory agents, metabolic diseases, disease diagnosis, etc., can solve the problem of unclear exact mechanism of chemokine-mediated events

Pending Publication Date: 2017-12-15
JYANT TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the precise mechanism of chemokine-mediated events remains unclear

Method used

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  • Anti-CXCL9, anti-CXCL10, anti-CXCL11, anti-CXCL13, anti-CXCR3 and anti-CXCR5 reagents used for inflammatory diseases
  • Anti-CXCL9, anti-CXCL10, anti-CXCL11, anti-CXCL13, anti-CXCR3 and anti-CXCR5 reagents used for inflammatory diseases
  • Anti-CXCL9, anti-CXCL10, anti-CXCL11, anti-CXCL13, anti-CXCR3 and anti-CXCR5 reagents used for inflammatory diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0226] Example 1: Upregulation of chemokines and their receptors in inflammatory diseases

[0227] Materials and methods

[0228]The protein sequences of the chemokines used herein are recorded in NIH-NCBI GenBank as follows: (1) CXCR1 (ACCESSION #NP 000625), (2) CXCR2 (ACCESSION #NP001548), (3) CXCL1 (ACCESSION #NP001502), ( 4) CXCL2 (ACCESSION#NP 002080), (5) CXCL3 (ACCESSION#NP 002081), (6) CXCL5 (ACCESSION#NP 002985), (7) CXCL6 (ACCESSION#NP 002984), (8) CXCL7 (ACCESSION# NP002695), (9) CXCL8 (IL-8, ACCESSION#NP 000575), (10) CXCR4 (ACCESSION#NP 003458), (11) CXCL12 (ACCESSION#NP 000600), (12) CXCR5A (ACCESSION#NP 116743) 、(13)CXCR5B(ACCESSION#NP001707),(14)CXCL13(ACCESSION#NP006410),(15)CXCR6(ACCESSION#NP006555),(16)CXCL16(ACCESSION#NP071342),(17)CCL16(ACCESSION#NP071342),(17)CCL16(ACCESSION#NP006410), 004581), (18) CCL25 (ACCESSION#NP_005616.2), (19) CCL25-1 (ACCESSION#NP005615), (20) CCL25-2 (ACCESSION#NP 683686), (21) CX3CR1 (ACCESSION#NP 001328) and (22) CX3CL1 (...

Embodiment 2

[0246] Example 2: mRNA expression of IFN-γ, CXCL10, MIG, I-TAC, and CXCR3 in murine colitis

[0247] figure 1 The mRNA expression of IFN-γ, CXCL10, MIG, I-TAC and CXCR3 during murine colitis is shown. IL-10 reared from cages with a C57BL / 6 background - / -Mice were removed from the laminar flow barrier to naturally develop colitis. After sacrifice, total RNA was isolated from the colon or mesenteric lymph nodes of mice before the onset of colitis (sterile conditions, blank rectangular bars) and after colitis onset (filled rectangular bars). IFN-γ, IP-10, MIG, I-TAC and CXCR3 mRNA expression levels were determined following RT-PCT analysis capable of detecting greater than 20 copies of transcribed cDNA. exist figure 1 In, the Log of the transcript 10 Copies are expressed relative to the true copy of 18S rRNA.

[0248] Such as figure 1 showed that IL-10 in patients with colitis - / - Significant increases in CXCR3 and CXCL10 expression were observed in the inflamed colon o...

Embodiment 3

[0249] Example 3: Reception of CD45RB by adoptive transfer HI or CXCR3 + CD4 + TCRβxδ of T cells - / - in mice Histological Analysis of IBD

[0250] figure 2 Shows acceptance of CD45RB by adoptive transfer HI or CXCR3 + CD4 + TCRβxδ of T cells - / - Histological analysis of IBD in mice. CD45RB received from normal C57BL / 6 mice Lo - (Panel A), CD45RB Hi - (Panel B) or CXCR3 + -CD4 + TCRβxδ of T cells (Panel C) - / - 60X magnification of enteritis in mice. Transverse sections of the intestine showed differences in wall thickness, enlargement of the mucosal layer, crypt deformation, and leukocyte infiltration using hematoxylin-eosin stained 6 μm paraffin sections.

[0251] This analysis revealed that CXCR3, both composed of the CD45RB population, + CD4 + T cells in TCRβxδ - / - Induction of colitis was induced in mice (Panel C)

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Abstract

The invention discloses a method for detecting inflammatory diseases of a subject. The method comprises the following steps: (a) detecting the expression level of one or more inflammatory disease markers in a biological sample obtained from the subject; and (b) comparing the expression level of one or more inflammatory disease markers in the biological sample with the normal expression level of the one or more inflammatory disease markers, wherein the one or more inflammatory disease markers comprise one or more markers selected from a group composed of CXCL9, CXCL10, CXCL11, CXCL13, CXCR3 and CXCR5. The invention also discloses a method for monitoring the inflammatory disease treatment process of the subject and a kit for detecting the inflammatory diseases of the subject.

Description

[0001] This application is for the invention titled "anti-CXCL9, anti-CXCL10, anti-CXCL11, anti-CXCL13, anti-CXCR3 and anti-CXCR5 reagents for inflammatory diseases", the international application number is "PCT / US2012 / 044475", the Chinese national phase application number is "201280075410.1", and the application date is "June 27, 2012". The above application is incorporated herein by reference in its entirety. technical field [0002] This application mainly relates to the detection of inflammatory diseases. More specifically, the present application relates to methods for detecting inflammatory diseases using anti-chemokine and / or anti-chemokine receptor detection reagents. Background technique [0003] Despite recent advances in research related to inflammatory processes, methods for diagnosing and treating chronic inflammatory diseases remain largely unknown. This may be because the factors that initiate and maintain the inflammatory state in the host are many and com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/7158G01N2800/52A61K2039/505A61P3/10A61P29/00C07K16/24C07K2317/76
Inventor 詹姆斯·W·利拉德
Owner JYANT TECH
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