Method for acquiring predatory mite spermatophore

Abstract

The invention relates to a method for acquiring predatory mite spermatophore. The method is characterized by a method for artificially acquiring and picking spermatophore from phytoseiidae predatory mite capitulum in the mating process. The method comprises the following steps: acquiring male and female mites in the mating process; shaping male and female mites; separating male and female mites; and acquiring and storing spermatophore. All the operations, except for the shaping of the male and female mites in the mating process, are performed under the microscope. The invention utilizes a simple and smart method to acquire the predatory mite spermatophore; the acquisition for the spermatophore is capable of supplying material source for the research on the male mite sperm; the invention also can supply reference for the acquisition for the other colony predatory mite spermatophore.

Description

Technical field [0001] The invention relates to a method for obtaining the essence pack of predatory mites of the Phytoseiaceae family, and belongs to the field of basic biological research of predatory mites. Background technique [0002] Phytoseiidae predator mites are dioecious animals. They must be mated and fertilized by male and female mites before they lay eggs and breed their offspring. Both male and female offspring develop from fertilized eggs. [0003] Existing information describes the mating of female and male mites of Phytoseiidae predator mites to form fertilized eggs: the sperm of male mites are stored in the semen before entering the female mites, and the female mites stuff the semen into the mating holes of female mites (Reference: Liang Lairong, 1979, Plant Protection, How do mites live), the sperm is slowly released from the seminal vesicle, transferred to the fertilized vesicle and combined with the egg to form a fertilized egg. However, there is no report on ...

AI Technical Summary

Problems solved by technology

[0004] However, due to the fact that the individual predatory mites of Phytoseiidae are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only 1 hour The size of the spermatozoa is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites, which are in close contact with each other. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data

Therefore, it is very difficult to obtain spermatozoa, and it is necessary to develop a method to obtain the spermatozoa of predatory mite