Method for determining gastrointestinal dysbiosis

Abstract

The present invention provides a method for determining the likelihood of a gastrointestinal dysbiosis in an individual, the method comprising providing a test data set, wherein the test data set comprises at least one microbiota profile, the microbiota profile being a distribution of relative levels of a plurality of microorganisms or groups of microorganisms in a sample from the gastrointestinal tract of said individual and wherein each level of each microorganism or group of microorganisms is a distribution element of said test data set, to said The test data set applies at least one loading vector determined from a latent variable within a horizontal distribution of the plurality of microorganisms or groups of microorganisms in corresponding gastrointestinal samples from a plurality of normal individuals, thereby generating a first projection data set, to applying a transposed version of the at least one loading vector to the first projection data set, thereby producing a second projection data set, comparing the test data set with the second projection data set and combining the second projection data set and the difference between the corresponding distribution elements of the test data set, and combining the difference with the plurality of microorganisms or microorganisms in the corresponding gastrointestinal samples from a plurality of normal individuals and / or individuals with dysbiosis comparing the normal to dysbiotic threshold values ​​determined by the correspondence analysis of the cohorts, applying at least one eigenvalue to said first projection data set, said eigenvalues ​​being determined by said at least one loading vector, and combining each The resulting values ​​of a distribution element and combining the combined value with the flora normal to Dysbiotic thresholds are compared, wherein a microbiota profile having said combined difference or said combined result above said respective normobacterial to dysbiotic thresholds is indicative of the likelihood of dysbiosis.

Description

technical field [0001] The present invention relates to the diagnosis, monitoring and / or characterization of diseases and conditions associated with perturbations in the microbiota of the gastrointestinal (GI) tract. Rather, the present invention provides a means by which the state of the gastrointestinal microbiota can be assessed and can be easily implemented, reliable and robust and flexible enough to be used with any technique used to measure microbial levels in gastrointestinal samples Deviating from the normal state (normal flora) is determined in a way that the flora is out of balance. In more specific embodiments, such deviations can be to a quantifiable extent, and thus the present invention provides a means of determining the extent of gastrointestinal dysbiosis, which in turn can indicate the severity of an associated disease or condition or can be used to monitor associated Progression of a disease or condition or symptoms of a related disease or condition. Back...

AI Technical Summary

Problems solved by technology

Clearly, perturbation of the GI microbiota results in deficiencies of certain microorganisms and / or excesses of others and / or reduced diversity and this results in a shift or imbalance in the relative activity of each microbial population

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