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Method for extracting alpha-glucosidase inhibitor from carambola root

A technology for glucosidase and inhibitors, applied in the field of preparation of α-glucosidase inhibitors, can solve problems such as vomiting, and achieve the effects of low price, strong operability, and reasonable preparation process

Active Publication Date: 2020-04-07
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since acarbose and others are synthetic drugs, nausea, vomiting and other gastrointestinal adverse reactions may occur when taken orally

Method used

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  • Method for extracting alpha-glucosidase inhibitor from carambola root
  • Method for extracting alpha-glucosidase inhibitor from carambola root
  • Method for extracting alpha-glucosidase inhibitor from carambola root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] Example: Separation and purification of a novel α-glucosidase activity inhibitor from carambola root water extract

[0038] Determination of the inhibitory effect of 3,4,5-trimethoxyphenol 1-O-[β-D-glucosyl-(1→6)-β-D-glucoside on α-glucosidase activity:

[0039] A: Determination principle: Colorless p-nitrophenol-α-D-glucoside (pNPG) is used as the reaction substrate, and yellow p-nitrophenol (pNP) can be generated after being hydrolyzed by α-glucosidase. The maximum absorption occurs at 405nm, and the inhibition of enzyme activity is indirectly determined by measuring the change of absorbance at 405nm.

[0040]

[0041] B: Determination system: Add 50 μL of each drug solution to be tested with a concentration of 2 mg / mL into the microplate plate, and set up three replicate wells for each concentration. In addition, drug control wells, blank reaction wells and blank control wells were set up. Add 50 μL of 0.26 U / mL α-glucosidase to the reaction well, add 50 μL phos...

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Abstract

The invention discloses a method for extracting an alpha-glucosidase inhibitor from averrhoa carambola root. The method comprises the following steps: (1) airing the averrhoa carambola root and smashing; (2) taking averrhoa carambola root coarse powder, putting into water, soaking, decocting and filtering, and repeating for three times, thus obtaining aqueous extract; concentrating; then adding ethanol, precipitating and staying overnight, carrying out suction filtration, taking supernatant, and concentrating, thus obtaining concentrated supernatant; (3) carrying out chromatographic column rough separation on the concentrated supernatant, and sequentially obtaining flow components 1-5; (4) taking a No.3 flow component, carrying out secondary chromatographic column separation, and sequentially obtaining flow components 6-12; (5) carrying out tertiary chromatographic column separation on the flow component 10, and sequentially obtaining flow components 13-21; (6) carrying out quaternary chromatographic column separation on the flow component 14, and sequentially obtaining flow components 22-28; and (7) carrying out fifth chromatographic column separation on a flow component 23, thus obtaining the target product alpha-glucosidase inhibitor. An in vivo experiment proves that the target product has an inhibiting effect on activity of alpha-glucosidase, and the inhibitory effect on the alpha-glucosidase is close to that of a first-line drug acarbose used for controlling type II diabetes at present.

Description

technical field [0001] The invention relates to the field of preparation of alpha-glucosidase inhibitors, in particular to a method for extracting alpha-glucosidase inhibitors from carambola roots. Background technique [0002] Diabetes mellitus is a disease of the endocrine system characterized by hyperglycemia, which is mainly caused by the disorder of glucose metabolism caused by relative or absolute insufficiency of insulin in the body. Among them, type II diabetes accounts for more than 90%. The mechanisms of various hypoglycemic substances reported today are different, and they are often multi-target, multi-effect, and multi-pathway comprehensive effects, in which by inhibiting the activity of α-glucosidase, the digestion time of carbohydrates is delayed, and the food intake is reduced. Post-hyperglycemia, thereby preventing and treating diabetes and reducing diabetic complications, is considered to be an ideal way to control type II diabetes. Clinically used α-glucos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/08A61P3/10
CPCC07H1/08C07H15/203
Inventor 廖彭莹李承曼陈慧萍唐丽娟陈颖燃
Owner GUANGXI UNIV OF CHINESE MEDICINE
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