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Apios americana Medikus flower polysaccharide and preparation method thereof

A technology of bean taro flower and bean taro, applied in a method and the field of polysaccharide obtained, can solve problems such as complex structure, achieve high purity, great application value, and low energy consumption

Inactive Publication Date: 2017-11-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The sugar chain combination of plant polysaccharides is mainly β-1,3 or β-1,6 bonds, and some polysaccharides also have branched chains, and the overall structure is more complex

Method used

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  • Apios americana Medikus flower polysaccharide and preparation method thereof
  • Apios americana Medikus flower polysaccharide and preparation method thereof
  • Apios americana Medikus flower polysaccharide and preparation method thereof

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Embodiment 1

[0062] Embodiment 1, a kind of preparation method of taro flower polysaccharide, carries out following steps successively:

[0063] 1), preparation of taro flower polysaccharide crude product:

[0064] According to the solid-liquid ratio of 1g:5ml, weigh 100g of taro flower and add 500mL of distilled water, extract in 90°C water bath for 80min (enzyme inactivation is also achieved during extraction), then beat, and then centrifuge (4000r / min for 15min), and Suction filter the supernatant obtained by centrifugation and collect the filtrate;

[0065] Use the filter residue obtained by centrifugation to replace the taro flower, repeat the above process of adding distilled water, then extracting (90°C water bath for 2 hours), beating, and centrifuging twice, and combine the filtrate obtained by centrifuging and suctioning for three times to concentrate to the original volume. 1 / 5 of the concentrated solution.

[0066] Under the condition of stirring, add ethanol (pure ethanol) t...

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Abstract

The invention discloses Apios americana Medikus flower polysaccharide and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a crude product of Apios americana Medikus flower polysaccharide, namely mixing Apios americana Medikus flowers with distilled water, sequentially carrying out extraction, pulping, centrifugation and concentration, so as to obtain concentrated liquid, adding ethanol into the obtained concentrated liquid until the final concentration of the ethanol is 80+ / -5%, standing at 4+ / -1 DEG C for 24+ / -2h, and collecting precipitates, so as to obtain the crude product of the Apios americana Medikus flower polysaccharide; and (2) purifying the Apios americana Medikus flower polysaccharide, namely carrying out activated carbon decoloration and Sevage-method deproteinization on the Apios americana Medikus flower polysaccharide. The prepared Apios americana Medikus flower polysaccharide contains a beta-glucosidic bond, a pyranose ring, glucose, fructose and xylose, and the molar ratio of glucose to fructose and to xylose is 1.79 to 1 to 0.03.

Description

technical field [0001] The invention relates to the field of polysaccharide extraction, in particular to a method for extracting and purifying polysaccharides from bean taro flowers and the obtained polysaccharides. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, Jinhua, Longyou, Wenzhou and other places. Studies have shown that different parts of taro (flowers, vines, leaves, tubers) are rich in free amino acids, soluble proteins, polysaccharides, saponins, flavonoids, isoflavones, vitamin C, vitami...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/0003
Inventor 郑晓冬楚强陈鹏华
Owner ZHEJIANG UNIV
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