Ready-to-use high-flux three-dimensional drug screening model and preparation method

Abstract

The invention provides a ready-to-use high-flux three-dimensional drug screening model and a preparation method. The model structurally comprises a micro-channel structure and a platform structure, wherein the micro-channel structure simulates a natural organ vascular structure and is made of biological hydrogel coated cells, the platform structure is only made of biological hydrogel, and the two structures are crosslinked to form the three-dimensional drug screening model. The preparation method comprises steps as follows: a three-dimensional computer model adopts an organ microstructure vascular structure is reconstructed firstly, a resin mold similar to the natural organ vascular structure is prepared with a 3D printing technique, and the micro-channel structure of the resin mold is formed in hydrogel through silicone rubber forming; the hydrogel is prepared from a serum-free rapid cell freezing medium and serum which are blended in proportion to dissolve a biological material and subjected to crosslinking treatment. The ready-to-use three-dimensional drug screening model simulates the in-vivo tissue physiological and biochemical environment and process to the maximum extent, the consistency of drug screening models is kept, and the drug screening accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of drug screening models, in particular to a ready-to-use high-throughput three-dimensional drug screening model and a preparation method. Background technique [0002] Drug development is a broad field of research that largely relies on monolayer cell culture and animal models as preclinical drug screening and testing platforms, but most of the failures of clinical trials are due to the lack of previous monolayer cell experiments or animal experiments. due to efficiency or accuracy. The three-dimensional drug screening model can provide a more accurate three-dimensional structure representing in vivo tissues, reducing the risk of failure of animal experiments and clinical trials, as well as the instability and uncertainty of existing high-throughput drug screening in vitro. Contents of the invention [0003] In order to overcome the shortcomings of the above-mentioned prior art, the object of the present...

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Problems solved by technology

[0002] Drug development is a broad field of research that largely relies on monolayer cell culture and animal models as preclinical drug screening and testing platforms, but most of the failures of clinical trials are due to the lack of previous monolayer cell experiments or animal experiments. due to efficiency or accuracy

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