Cell culture counting device

Abstract

The invention discloses a cell culture counting device. The device comprises a culture tank used for culturing cells, and the culture tank is communicated with a counting pond through a peristaltic pump. The counting pond comprises two transparent cover plates connected to a pipeline, a gap between the transparent cover plates only allows a layer of cells to pass through, and therefore the number of the cells in sight is the number of all the contained cells; the counting pond is shot through a digital microscope which is supported through a mechanical arm, data is transmitted to a central data processor to conduct image processing counting analysis. The number of the cells in a sample is obtained automatically according to shot image information, and the total number of the cells in the culture tank is obtained through a series of conversions. According to the cell counting method, manual observation counting is not needed, the labor intensity is lowered, and the result obtained by combining the image processing analysis technology is more accurate and reliable; cell samples used for detection are taken out of the culture tank directly without making contact with the outside world, after detection is completed, the cell samples can be reversely delivered back to the culture tank to be cultured continuously, and waste is avoided.

Description

technical field [0001] The invention relates to the technical field of cell culture, and further relates to a cell culture counting device. Background technique [0002] Cell culture is a method of studying animal cell behavior established at the beginning of the 20th century. Cells are taken out from tissues in the body, and the growth environment in the body is simulated. its structure and function. Cell preparation is the process of using cell culture protocols to produce high-purity cell final products for use in scientific research and clinical fields. [0003] Cell counting refers to calculating the total number of cells in the cell suspension by measuring the number of cells in a certain volume of suspension when the cells in the cell suspension to be tested are evenly distributed. Cell count is the most direct indicator to reflect the growth and proliferation of cells in the process of cell preparation, and it is an essential link in drawing cell growth curves. Ce...

AI Technical Summary

Problems solved by technology

[0005] Regardless of manual counting using a microscope or automatic counting by an automated cell counting analyzer, experienced professional technicians must complete the operation. The cell counting process is divided into two steps. The collection of cells for counting and cell culture are independent processes, and it is difficult to guarantee The uniformity of the sample, the counting process after collecting the sample has a time difference, which cannot avoid the risk of errors or contamination during the collection, transportation, and counting processes, and cannot reflect the real growth and proliferation of cells in time

For some precious cell types, there is a risk of contamination during counting, and it is difficult to recover after the counting is completed, so it can only be discarded, resulting in loss and waste

Counting results require a lot of manual intervention, which brings pollution risks. At the same time, the counting results are also affected by the accumulation of technical experience of quality inspection technicians and human subjective factors.

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