Resistant protein and coding gene of bacterial blight

Abstract

The invention belongs to the technical field of genetics and discloses a resistant protein and a coding gene of bacterial blight. The cDNA sequence of the bacterial blight resistant protein coding gene is SEQ ID NO:1; and the amino acid sequence of the bacterial blight resistant protein is SEQ ID NO:2. A construction method of the bacterial blight resistant protein coding gene comprises the following steps of performing homologous searching on an oryza granulata transcriptome CDS library and obtaining an Om46055 cDNA sequence; designing a primer according to the sequence of the Om46055, and adopting a 3' / 5'RACE-PCR method to clone the overall length of the Om46055 from oryza granulata; and building an overexpression vector and a gene function. A new disease-resistant gene which is dominant and is high in bacterial blight resistance and a coding protein thereof are obtained, disease-resistant gene resources of cultivated rice are enriched, and the resistant protein and the coding gene have important application value in cultivating new disease-resistant breed and preventing and controlling bacterial blight.

Description

technical field [0001] The invention belongs to the technical field of genetics, and in particular relates to a bacterial blight resistance protein and a coding gene. Background technique [0002] Rice plays a pivotal role in world food security. Rice bacterial blight (Bacterial Blight) is the most serious bacterial disease in rice production in the world, which can cause 20-30% yield loss, or even grain failure. The cloning and application of superior bacterial blight resistance genes is the most economical and effective measure to control the disease. However, the application of identified or cloned bacterial blight resistance genes in disease-resistant breeding is very limited, such as Xa1, Xa10, Xa11 and Xa18 have narrow antibacterial spectrum, and are not resistant to most bacterial blight strains in my country ( Bhasin et ai., 2012), although disease resistance genes such as xa5, xa8, xa13, xa19 and xa20 have a broad spectrum of resistance, they are recessive genes an...

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Problems solved by technology

[0004] To sum up, the problems existing in the existing technology are: the bacterial blight resistance gene resources currently used in rice disease resistance breeding are mainly Xa21 and Xa23, the genetic basis of resistance is narrow, and the resistance is easy to lose. There are potentially huge risks to rice production and world food security; Oryza sativa has a GG genome, which is far from cultivated rice, and it is impossible to transfer and utilize the excellent disease resistance genes in Oryza sativa through conventional hybridization methods

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