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Medium applicable to Lactobacillus acidophilus and preparation method thereof

The technology of Lactobacillus acidophilus and culture medium is applied to the culture medium of Lactobacillus acidophilus and the field of preparation thereof, and can solve the problems such as long time for Lactobacillus acidophilus to enter the stable phase, low number of viable bacteria of Lactobacillus acidophilus, and the like, Achieve the effect of shortening the time and increasing the number of viable bacteria

Active Publication Date: 2017-09-29
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of live bacteria of Lactobacillus acidophilus obtained from this medium is low, and the time for Lactobacillus acidophilus to enter the stationary phase is long

Method used

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  • Medium applicable to Lactobacillus acidophilus and preparation method thereof
  • Medium applicable to Lactobacillus acidophilus and preparation method thereof
  • Medium applicable to Lactobacillus acidophilus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 A kind of medium suitable for Lactobacillus acidophilus

[0030] The medium is composed of the following components by weight: distilled water 1000ml, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, yeast extract 20g / L, dipotassium hydrogen phosphate 2g / L, diammonium hydrogen citrate 2g / L L. Beef extract 10g / L, agar 14g / L, peptone 10g / L, Tween 801g / L, tomato juice 150g / L, sodium acetate 15g / L, glucose 20g / L and dandelion extract 0.1g / L.

[0031] Preparation:

[0032] A) Preparation of tomato juice: take fresh tomatoes, wash and slice them, add 1 times the amount of tomato purified water, blanch for 10 minutes, squeeze the juice, filter, adjust the pH to 6.3, sterilize at 115°C for 15 minutes, and store in the refrigerator. spare;

[0033] B) Preparation of dandelion extract: Weigh 20 g of dandelion medicinal material, pulverize, add 500 mL of water and decoct twice for 0.5 h each time, filter, combine the filtrates, concentrate and dry, and pulver...

Embodiment 2

[0035] Embodiment 2, a kind of medium suitable for Lactobacillus acidophilus

[0036] The medium is composed of the following components by weight: 1500ml of distilled water, 0.2g / L of magnesium sulfate, 0.1g / L of manganese sulfate, 10g / L of yeast extract, 1.0g / L of dipotassium hydrogen phosphate, 1.0 g / L of diammonium hydrogen citrate g / L, beef extract 5g / L, agar 10g / L, peptone 5g / L, Tween 800.5g / L, tomato juice 100g / L, sodium acetate 7.5g / L, glucose 10g / L and dandelion extract 0.04g / L.

[0037] The preparation method refers to Example 1.

Embodiment 3

[0038] Embodiment 3, a kind of culture medium suitable for Lactobacillus acidophilus

[0039] The medium is composed of the following components by weight: distilled water 2000ml, magnesium sulfate 1.0g / L, manganese sulfate 0.5g / L, yeast extract 40g / L, dipotassium hydrogen phosphate 4.0g / L, diammonium hydrogen citrate 4.0 g / L, beef extract 20g / L, agar 20g / L, peptone 20g / L, Tween 802g / L, tomato juice 200g / L, sodium acetate 30g / L, glucose 40g / L and dandelion extract 0.2g / L .

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Abstract

The invention specifically relates to a medium applicable to Lactobacillus acidophilus and a preparation method thereof, belonging to the field of biotechnology. The medium comprises, by weight, 1000 to 2000 ml of distilled water, 0.2 to 1.0 g / L of magnesium sulfate, 0.1 to 0.5 g / L of manganese sulfate, 10 to 40 g / L of yeast extract, 1.0 to 4.0 g / L of dipotassium hydrogen phosphate, 1.0 to 4.0 g / L of diammonium hydrogen citrate, 5 to 20 g / L of beef extract, 10 to 20 g / L of agar, 5 to 20 g / L of peptone, 0.5 to 2 g / L of Tween-80, 50 to 200 g / L of tomato juice, 7.5 to 30 g / L of sodium acetate, 10 to 40 g / L of glucose and 0.04 to 0.2 g / L of dandelion extract. The medium is especially applicable to culture of Lactobacillus acidophilus, can substantially increase the live-bacterium number of Lactobacillus acidophilus, and obviously shortens time before a stationary phase of Lactobacillus acidophilus; and due to addition of natural factors, prepared Lactobacillus acidophilus contains a great number of beneficial metabolites.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a culture medium suitable for Lactobacillus acidophilus and a preparation method thereof. Background technique [0002] Lactobacillus acidophilus belongs to the genus Lactobacillus, which can produce lactic acid by fermenting carbohydrates. It is widely found in the intestinal tract of humans and animals, and is important for human health, especially for maintaining the normal physiological functions of the gastrointestinal tract. It is an important beneficial microbial flora in the intestinal tract. [0003] Due to the special requirements for the growth conditions of Lactobacillus acidophilus, the content of live bacteria in Lactobacillus acidophilus products currently on the market is relatively low. For this reason, people have been actively exploring substances that can promote its growth, so as to increase the viable count of Lactobacillus acidophilus. At the same...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/23
CPCC12N1/20
Inventor 管淑玉徐硕超李洋梁生旺王淑美
Owner GUANGDONG PHARMA UNIV
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