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Preparation method capable of prolonging shelf life of enzyme

A shelf life and enzyme technology, applied in the field of enzymes, can solve problems affecting products, food nutrition loss, chemical preservatives that do not meet product positioning, etc., and achieve the effect of solving shelf life problems

Inactive Publication Date: 2017-09-19
张萌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Changing any of these can affect the shelf life of the product—either positively or negatively
At present, there are two commonly used ways to achieve shelf life. One is to use heat sterilization to achieve product shelf life. The disadvantage of this method is that heat sterilization will cause loss of food nutrition; the other is to use preservatives to achieve shelf life. , the disadvantage of this method is that most preservatives are chemical products, coupled with people's demands for health, food with chemical preservatives is alienated by consumers
[0004] Enzyme products are a type of food that has been fermented by microorganisms and contains biologically active ingredients. They are favored by consumers due to their health care effects. However, due to the rich nutrition of enzyme products, how to prolong their shelf life has become the focus of the industry. In order to maximize the retention of enzymes For the active ingredients in the product, it is obviously not suitable to use the heat sterilization process to improve the shelf life, but as an enzyme product for health products, adding chemical preservatives is obviously not in line with the positioning of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method for extending the shelf life of ferment, comprising the following steps:

[0022] 1) Raw material processing: citrus, apple, grape, strawberry, pineapple, banana, peach, pear, apricot, melon, watermelon, coconut and blueberry are used as raw materials, washed and crushed separately, and mixed to obtain fruit pulp.

[0023] 2) Fruit pulp treatment: quickly cool down the fruit pulp obtained in step 1) to 18° C., then perform homogenization treatment, and adjust the pH to 3.5.

[0024] 3) The first stage of fermentation: adding the mixed bacteria of Lactobacillus acidophilus and Lactobacillus plantarum (the amount of mixed bacteria added is 5wt% of the total weight of raw materials, and the mass ratio of Lactobacillus acidophilus and Lactobacillus plantarum is 1:2) Step 2) Fermentation is carried out in the homogenized fruit pulp at 28° C. until the sugar content in the fermentation broth is as low as 10 g / L, then the fermentation is stopped.

[0025]...

Embodiment 2

[0029] A preparation method for extending the shelf life of ferment, comprising the following steps:

[0030] 1) Raw material processing: citrus, apple, grape, strawberry and pineapple are used as raw materials, each raw material is washed, crushed and processed separately, and mixed to obtain fruit pulp.

[0031] 2) Fruit pulp treatment: quickly cool down the fruit pulp obtained in step 1) to 20° C., then perform homogenization treatment, and adjust the pH to 3.0.

[0032] 3) The first stage of fermentation: the mixed bacteria of Lactobacillus casei and Lactobacillus fermentum (the addition of mixed bacteria is 5wt% of the total weight of raw materials, and the mass ratio of Lactobacillus casei and Lactobacillus fermentum is 1:2.2) is added to step 2 ) in the homogenized fruit pulp for fermentation at 30°C until the sugar content in the fermentation broth is as low as 10g / L, then the fermentation is stopped.

[0033] 4) The second stage of fermentation: add taurine, selenium...

Embodiment 3

[0037] A preparation method for extending the shelf life of ferment, comprising the following steps:

[0038] 1) Raw material processing: use pineapples, bananas, peaches, pears, apricots, melons, watermelons, coconuts and blueberries as raw materials, wash each raw material, crush them separately, and mix them to obtain fruit pulp.

[0039] 2) Fruit pulp treatment: quickly cool down the fruit pulp obtained in step 1) to 20° C., then perform homogenization treatment, and adjust the pH to 3.5.

[0040] 3) The first stage of fermentation: adding the mixed bacteria of Lactobacillus acidophilus and Lactobacillus plantarum (the amount of mixed bacteria added is 8wt% of the total weight of raw materials, and the mass ratio of Lactobacillus acidophilus and Lactobacillus plantarum is 1:2.1) Step 2) Ferment in the homogenized fruit pulp at 26° C. until the sugar content in the fermentation broth is as low as 10 g / L, then stop the fermentation.

[0041] 4) The second stage of fermentat...

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PUM

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Abstract

The invention provides a preparation method capable of prolonging shelf life of enzyme. The preparation method comprises the following steps: 1) raw material processing: fruit pulp is prepared from one or more of citrus, apple, grape, strawberry, pineapple, banana, peach, pear, apricot, muskmelon, watermelon, coconut and blueberry as raw materials; 2) fruit pulp processing; 3) first-stage fermentation: lactobacillus acidophilus and lactobacillus plantarum mixed bacteria or lactobacillus casei and lactobacillus fermentum mixed bacteria are added to the fruit pulp homogenized in the step 2) for fermentation; 4) second-stage fermentation: taurine, selenium, proline, proline, inositol and brown granulated sugar are added to fermentation broth in the step 3), and then the mixture is inoculated with lactococcus lactis subsp lactis and Lactococcus lactis subsp. Cremoris mixed bacteria for fermentation; 5) filtration and concentration. With adoption of the method, microorganisms are induced to produce nisin to serve as natural ablastin in an enzyme preparing process, and the problem about the shelf life of an enzyme product can be solved well.

Description

technical field [0001] The invention belongs to the technical field of enzymes, in particular to a preparation method for extending the shelf life of enzymes. Background technique [0002] Enzymes exist in all living animals and plants, and are an essential substance for maintaining normal body functions, digesting food, repairing tissues and other life activities. With the growth of age, food safety, living environment and other issues, the enzyme content in the human body is decreasing year by year, the body will reduce the body's metabolic rate due to the loss of enzymes, prone to problems such as sub-health and chronic diseases, showing an aging trend year by year . If there is no enzyme, the biochemical reaction will not be able to proceed, the five major nutrients will become useless to the body, and the phenomenon of life will stop. Supplementing enzymes can make all life activities in the body proceed in an orderly manner, and keep our body alive at all times. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00
CPCA23L33/00
Inventor 张萌
Owner 张萌
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